吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (04): 698-704.doi: 10.13481/j.1671-587x.20170407

• 基础研究 • 上一篇    下一篇

复方麦鹿芪人参制剂主要成分变化及其在肝癌H22荷瘤小鼠体内的抗肿瘤作用

王婷婷1,2, 郜玉钢1,3, 臧埔1, 赵岩1, 何忠梅1, 祝洪艳1, 张连学1,3   

  1. 1. 吉林农业大学中药材学院生物技术教研室, 吉林 长春 130118;
    2. 解放军第208医院康复科, 吉林 长春 130062;
    3. 吉林省人参工程技术研究中心, 吉林 长春 130118
  • 收稿日期:2016-12-15 出版日期:2017-07-28 发布日期:2017-08-01
  • 通讯作者: 郜玉钢,教授,硕士研究生导师(Tel:0431-86988016,E-mail:gaoyugang_2006@163.com);张连学,教授,博士研究生导师(Tel:0431-84533358,E-mail:zlxbooksea@163.com) E-mail:gaoyugang_2006@163.com;zlxbooksea@163.com
  • 作者简介:王婷婷(1987-),女,吉林省长春市人,在读医学硕士,主要从事肿瘤药理学方面的研究。
  • 基金资助:
    国家科技部科技支撑计划项目资助课题(2016YFC0500300);吉林省科技厅科研基金资助课题(20140311050YY,20140307012YY,20170204017YY);国家教育部大学生创新创业训练计划项目资助课题(201610193025)

Changes of main components of compound Mailuqishen and its anti-tumor effect in H22 tumor-bearing mice

WANG Tingting1,2, GAO Yugang1,3, ZANG Pu1, ZHAO Yan1, HE Zhongmei1, ZHU Hongyan1, ZHANG Lianxue1,3   

  1. 1. Department of Biotechnology, School of Traditional Chinese Medicine, Jilin Agriculture University, Changchun 130118, China;
    2. Department of Rehabilitation, No. 208 Hospital of PLA, Changchun 130062, China;
    3. Center of Ginseng Engineering Technology Research, Jilin Province, Changchun 130118, China
  • Received:2016-12-15 Online:2017-07-28 Published:2017-08-01

摘要: 目的:分析复方麦鹿芪人参制剂配伍后主要成分的变化,探讨其对肝癌H22荷瘤小鼠的体内抗肿瘤作用。方法:采用HPLC法测定复方麦鹿芪人参制剂中人参皂苷和氨基酸的含量,苯酚硫酸法测定复方麦鹿芪人参制剂中多糖的含量。将144只雌性昆明小鼠随机分为正常对照组,模型组,阳性药组,低、中、高剂量复方麦鹿芪人参组,人参羹组,人参原粉组和鹿角脱盘原粉组,每组16只。除正常对照组外,其余8组构建肝癌H22荷瘤小鼠模型,造模后次日给药,连续给药10d后测定各组小鼠瘤质量,计算各组小鼠肿瘤抑制率、脾脏指数和胸腺指数,HE染色法观察肿瘤组织和脾脏组织的形态表现,流式细胞术检测肿瘤细胞凋亡率。结果:以人参和鹿角脱盘单味生药量计,复方麦鹿芪人参制剂中人参皂苷、多糖和氨基酸含量明显高于单味药(P<0.05)。与模型组比较,各剂量给药组小鼠肿瘤抑制率明显增加(P<0.01),各剂量复方麦鹿芪人参组小鼠脾脏指数和胸腺指数明显升高(P<0.01),肿瘤细胞凋亡率明显升高(P<0.05)。与模型组比较,各剂量复方麦鹿芪人参组小鼠肿瘤组织被破坏,细胞排列疏松,形状不规则,可见坏死灶;脾脏组织白髓结构逐渐趋于正常,边缘区可辨,淋巴细胞排列密集。结论:复方麦鹿芪人参制剂中皂苷、多糖和氨基酸含量与单味药相比均明显增加,且其抗肿瘤作用较单味药更强。

关键词: 氨基酸, 皂苷类, 人参, 鹿角脱盘, 多糖

Abstract: Objective: To determine the changes of the main components after compatibility of compound Mailuqishen(MLQS), and to explore its anti-tumor effect in the H22 tumor-bearing mice.Methods: The contents of ginsenoside and amino acid in MLQS were detected by HPLC, and the content of polysaccharide was detected by phenol-sulfuric acid. A total of 144 female Kunming mice were randomly divided into normal control group, model group, positive control group, low dose of MLQS group, medium dose of MLQS group, high dose of MLQS group, ginseng geng group, ginseng group and antler plate group (n=16). Except normal control group, the mice in the other eight groups were used to establish the H22 tumor-bearing mouse models, then the mice were treated with drugs at next day. The tumor weights, inhibitory rates of tumor, spleen and thymus indexes of the H22 tumor-bearing mice were detected 10 d after administration. The morphological changes of tumor and spleen tissue were examined by HE staining, and the apoptotic rates of H22 tumor cells were tested by flow cytometry.Results: As calculation with the ginseng and antler plate single herb, the contents of ginsenosides, polysaccharides and amino acids in MLQS were significantly higher than those of single herbs (P<0.05). Compared with model group, the inhibitory rates of tumor in various administration groups were significantly increased(P<0.01); the spleen indexes and thymus indexes of the mice in different doses of MLQS groups were significantly increased(P<0.01); the apoptotic rates of tumor cells were markedly increased(P<0.05). Compared with model group, the tumor tissue of the mice in various administration groups was destroyed, the cells were sparse and irregular, and the tumor presented necrotic lesions; the morphology of spleen tissue was normal with discernible fringe, and the lymphocytes arranged densely.Conclusion: The contents of ginsenosides, polysaccharides, and amino acids in compound MLQS are significantly increased compared with those of single herbs, and its anti-tumor effect is stronger than the single herbs.

Key words: polysaccharide, panax ginseng, antler plate, amino acid, saponins

中图分类号: 

  • R284