Notch 1对舌麟状细胞癌Tca8113细胞表皮生长因子受体表达的影响及其意义

doi:10.13481/j.1671-587x.20170410

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (04): 715-719.doi: 10.13481/j.1671-587x.20170410

Notch 1对舌麟状细胞癌Tca8113细胞表皮生长因子受体表达的影响及其意义

李浩渤¹, 杜勇², 陈勇¹, 李文静¹

1. 河北医科大学第二医院口腔内科, 河北石家庄 050000;
2. 河北省沧州市中心医院口腔科, 河北沧州 061014

收稿日期:2016-10-12 出版日期:2017-07-28 发布日期:2017-08-01
通讯作者: 李浩渤,副主任医师(Tel:0311-66002729,E-mail:haobobrother12@163.com) E-mail:haobobrother12@163.com
作者简介:李浩渤(1975-),男,河北省石家庄市人,副主任医师,医学硕士,主要从事牙周种植和牙体牙髓病诊治方面的研究。
基金资助:
河北省卫计委医学科学研究重点项目资助课题（20160101）

Influence of Notch 1 in expression of EGFR in tongue squamous cell carcinoma Tca8113 cells

LI Haobo¹, DU Yong², CHEN Yong¹, LI Wenjing¹

1. Department of Oral Medicine, Second Hospital, Hebei Medical University, Shijiazhuang 050000, China;
2. Department of Stomatology, Cangzhou Central Hospital, Hebei Province, Cangzhou 061014, China

Received:2016-10-12 Online:2017-07-28 Published:2017-08-01

摘要/Abstract

摘要： 目的：将编码Notch 1胞內域（NICD）转染人舌麟状细胞癌（舌麟癌）Tca8113细胞，探讨Notch 1在Tca8113细胞中的作用及其对表皮生长因子受体（EGFR）表达的影响。方法：采用脂质体法将编码NICD的pRAMIC-IRES2-EGFP目的质粒和pIRES2-EGFP对照质粒分别转染人舌麟癌Tca8113细胞，实验分为目的质粒pRAMIC-IRES2-EGFP转染组、对照质粒pIRES2-EGFP转染组和非转染组。采用RT-PCR法、Western blotting法和免疫细胞化学法观察细胞中Notch 1mRNA及蛋白和EGFR mRNA及蛋白表达水平，MTT法检测Tca8113细胞的增殖活性并计算细胞存活率。结果：转染后，与非转染组和对照质粒pIRES2-EGFP转染组比较，pRAMIC-IRES2-EGFP目的质粒转染组Notch 1 mRNA和蛋白表达水平明显升高（P<0.05），而EGFR mRNA和蛋白表达水平明显降低（P<0.05），细胞存活率明显降低（P<0.05）。与非转染组比较，对照质粒pIRES2-EGFR转染组Notch 1 mRNA和蛋白表达水平、EGFR mRNA和蛋白表达水平及细胞存活率差异无统计学意义（P>0.05）。结论：Notch 1对舌麟癌Tca8113细胞增殖的影响可能与EGFR的表达下调有关。

关键词: 舌麟状细胞癌, Notch 1, 转染, 表皮生长因子受体

Abstract: Objective: To transfect the tongue squamous cell carcinoma Tca8113 cells with NICD, and to investigate the effects of Notch 1 on Tca8113 cells and the expression of epidermal growth factor receptor (EGFR) in tongue squamous cell carcinoma.Methods: The Notch plasmid pRAMIC-IRES2-EGFP encoding exogenous and control plasmid pIRES2-EGFP were transiently transfected into the Tca8113 cells by Lipofectamine^TM 2000. In the experiment, target plasmid pRAMIC-IRES2-EGFP transfection group, control plasmid pIRES2-EGFP transfection group and non-transfection group were set up. RT-PCR, Western blotting and immunocytochemical method were used to observe the mRNA and protein expression levels of Notch 1 and EGFR. The proliferation activities of Tca8113 cells were detected by MTT assay,and the survival rates were calculated.Results: Compared with non-transfection group and control plasmid pIRES2-EGFP transfection group, the mRNA and protein expression levels of Notch 1 in Tca8113 cells in pRAMIC-IRES2-EGFP group was significantly increased(P<0.05), the mRNA and protein expression levels of EGFR in Tca8113 cells were significantly decreased (P<0.05),and the proliferation rate of Tca8113 cells was significantly decreased(P<0.05) after transfection. There were no significant differences in the expression levels of Notch 1 and EGFR mRNA and protein and the proliferation rates in Tca8113 cells between non-transfection group and pRAMIC-IRES2-EGFP group (P>0.05).Conclusion: The effect of Notch 1 on the proliferation of Tca8113 cells may be related to down-ragulatoin of expression level of EGFR.

Key words: tongue squamous cell carcinoma, Notch 1, epidermal growth factor receptor, transfection

中图分类号:

R739.86

李浩渤, 杜勇, 陈勇, 李文静. Notch 1对舌麟状细胞癌Tca8113细胞表皮生长因子受体表达的影响及其意义[J]. 吉林大学学报(医学版), 2017, 43(04): 715-719.

LI Haobo, DU Yong, CHEN Yong, LI Wenjing. Influence of Notch 1 in expression of EGFR in tongue squamous cell carcinoma Tca8113 cells[J]. Journal of Jilin University Medicine Edition, 2017, 43(04): 715-719.

参考文献

[1] Duan Y, Zhang S, Wang L, et al. Targeted silencing of CXCR4 inhibits epithelial-mesenchymal transition in oral squamous cell carcinoma[J]. Oncol Lett,2016,12(3):2055-2061.
[2] Mao L. NOTCH mutations:multiple faces in human malignancies[J]. Cancer Prev Res (Phila),2015, 8(4):259-261.
[3] Kangsamaksin T, Tattersall IW, Kitajewski J. Notch functions in developmental and tumour angiogenesis by diverse mechanisms[J]. Biochem Soc Trans, 2014, 42(6):1563-1568.
[4] Kim HJ, Kim MJ, Kim A. The role of Notch1 signaling in anaplastic thyroid carcinoma[J]. Cancer Res Treat, 2017,49(2):509-517.
[5] Nusser-Stein S, Beyer A, Rimann I,et al.Cell-cycle regulation of NOTCH signaling during C. elegans vulval development[J]. Mol Syst Biol, 2012,8:618.doi:10.1038/msb.2012.51.
[6] Pellegrino MW, Farooqui S, Fr hli E, et al. LIN-39 and the EGFR/RAS/MAPK pathway regulate C. elegans vulval morphogenesis via the VAB-23 zinc finger protein[J]. Development, 2011, 138(21):4649-4660.
[7] Jouan-Hureaux V, Boura C, Merlin JL, et al. Modulation of endothelial cell network formation in vitro by molecular signaling of head and neck squamous cell carcinoma (HNSCC) exposed to cetuximab[J]. Microvasc Res, 2012,83(2):131-137.
[8] Ryott M, Wangsa D, Heselmeyer-Haddad K, et al. EGFR protein overexpression and gene copy number increases in oral tongue squamous cell carcinoma[J]. Eur J Cancer, 2009,45(9):1700-1708.
[9] Wu G, Wilson G, George J, et al. Modulation of Notch signaling as a therapeutic approach for liver cancer[J]. Curr Gene Ther, 2015,15(2):171-181.
[10] Miele L. Notch signaling[J]. Clin Cancer Res, 2006,12(4):1074-1079.
[11] Klinakis A, Lobry C, Abdel-Wahab O, et al. A novel tumour-suppressor function for the Notch pathway in myeloid leukaemia[J]. Nature, 2011, 473(7346):230-233.
[12] Huang WH, Liao WR, Sun RX. Astragalus polysaccharide induces the apoptosis of human hepatocellular carcinoma cells by decreasing the expression of Notch 1[J]. Int J Mol Med, 2016,38(2):551-557.
[13] Deng Y, Greenwald I. Determinants in the LIN-12/Notch intracellular domain that govern its activity and stability during caenorhabditis elegans vulval development[J]. G3:Genes Genomes Genetics, 2016,19:116.
[14] Hansson EM,Lendahl U,Chapman G,et al. Notch signaling in development and disease[J]. Semin Cancer Biol,2004,14(5):320-328.
[15] Masuda S, Kumano K, Shimizu K, et al. Notch l oncoprotein antagonizes TGF-beta/Smad-mediated cell growth suppression via sequestration of coactivator p300[J]. Cancer Sci, 2005,96(5):274-282.
[16] Gusenbauer S, Zanucco E, Knyazev P, et al. Erk2 but not Erk1 regulates crosstalk between Met and EGFR in squamous cell carcinoma cell lines[J]. Mol Cancer,2015,14(1):54.
[17] Shao Y, Sha XY, Bai YX, et al. Effect of A disintegrin and metalloproteinase 10 gene silencing on the proliferation, invasion and migration of the human tongue squamous cell carcinoma cell line TCA8113[J]. Mol Med Rep,2015,11(1):212-218.
[18] 黄红杰.Notch 1在人舌麟癌中的作用及其与表皮生长因子受体信号的交互作用[D].杭州:浙江大学,2009.
[19] Ye QF, Zhang YC, Peng XQ, et al. siRNA-mediated silencing of Notch-1 enhances docetaxel induced mitotic arrest and apoptosis in prostate cancer cells[J]. Asian Pac J Cancer Prev,2012,13(6):2485-2889.
[20] Wang X, Song N, Zhang Y, et al. Coexpression of c-Met and Notch-1 correlates with poor prognosis in resected non-small-cell lung cancer[J]. Tumour Biol, 2015,36(9):7053-7059.

Notch 1对舌麟状细胞癌Tca8113细胞表皮生长因子受体表达的影响及其意义

Influence of Notch 1 in expression of EGFR in tongue squamous cell carcinoma Tca8113 cells

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	李里, 宫亮, 吴玉斌. 沉默WNT4基因对稳定转染PAX2基因大鼠肾小管上皮细胞迁移和侵袭能力的影响[J]. 吉林大学学报(医学版), 2018, 44(06): 1212-1217.
[2]	刘相良, 纪伟, 李理, 赵颖华, 贾琳, 万志强, 陈晓, 李薇. 盐酸厄洛替尼片及耐药后化疗联合贝伐珠单抗注射液治疗肺腺癌1例报告并文献复习[J]. 吉林大学学报(医学版), 2017, 43(05): 1019-1024.
[3]	于秀艳, 张晓伟, 野丽莉, 李铤, 刘晓峰, 吴雪峰. hMAM联合MMP-9和C-erbB2 mRNA表达检测在乳腺癌外周血微转移诊断中的应用[J]. 吉林大学学报(医学版), 2017, 43(05): 1009-1014.
[4]	陈志华, 林素勇, 韩宏景, 苏小宝, 陈绍勤, 戴起宝. 慢病毒转染KISS1基因对人结直肠癌HCT116细胞增殖、侵袭和迁移能力的影响[J]. 吉林大学学报(医学版), 2017, 43(03): 577-581.
[5]	于海帆, 郭传辉, 李当当, 张虹亮, 耿爽, 杨占清, 郭斌, 岳占碰. 多效生长因子对小鼠子宫基质细胞蜕膜化的影响[J]. 吉林大学学报(医学版), 2016, 42(05): 855-859.
[6]	张敏, 崔运芹, 宋焕芳, 吕建祎, 徐笑红, 高基民. 表皮生长因子受体对苯并芘诱导的人支气管上皮细胞SIRT1活化的影响[J]. 吉林大学学报(医学版), 2016, 42(04): 731-736.
[7]	韩春耀, 张斌, 马雪, 刘明媛, 薛中原, 郝丽静, 葛树卿. 沉默α-catulin基因的人舌鳞状细胞癌TSCCA细胞模型的建立[J]. 吉林大学学报(医学版), 2016, 42(03): 481-485.
[8]	温剑平, 崔永生, 张雄基. 转染EGFRL858R的肺腺癌细胞中IL-6和VEGF表达水平及其意义[J]. 吉林大学学报(医学版), 2016, 42(03): 502-505.
[9]	李凤娥, 向国艳, 孔繁利, 郝峰, 杨紫嫣, 郑岩, 方芳. pEGFP-N1脂质体转染方法的优化及其应用[J]. 吉林大学学报(医学版), 2016, 42(03): 612-616.
[10]	邹喜, 陈金梅, 洪金省, 郭飞宝, 蓝林臻, 张纬建. 非小细胞肺癌患者EGFR-TKI靶向治疗前后肿瘤体积变化规律及其临床价值[J]. 吉林大学学报(医学版), 2016, 42(02): 345-350.
[11]	邵婉婷, 付彤, 武盼盼, 吴迪, 李嗣杰, 郑超, 范志民. Her-2阳性乳腺癌患者曲妥珠单抗联合新辅助化疗的效果评价及其预后影响因素分析[J]. 吉林大学学报(医学版), 2016, 42(02): 351-357.
[12]	张宝月, 张俊农. 表皮生长因子受体酪氨酸激酶抑制剂厄洛替尼联合山奈酚诱导卵巢癌细胞凋亡的协同机制[J]. 吉林大学学报(医学版), 2016, 42(01): 31-35.
[13]	李春梅, 张林, 侯艳红, 李楠. 潜在抑癌基因TXNIP对胃癌MKN-45细胞生物学特性的影响[J]. 吉林大学学报(医学版), 2015, 41(03): 588-592.
[14]	王子航, 张宇虹, 夏稻子, 礼广森, 武俊, 由悦, 黄冬梅, 薄华颖, 胡滨, 毛鑫. 超声靶向破坏微泡技术介导小鼠肝癌细胞株JNK1基因表达、细胞迁移和侵袭抑制[J]. 吉林大学学报(医学版), 2015, 41(01): 21-26.
[15]	齐亚灵，赵文杰，方艳秋，陈玉强，孟德欣，王伟群，李尧，李文 . Survivin-ASODN对肝癌SMMC-7721细胞凋亡的促进作用及其机制[J]. 吉林大学学报(医学版), 2014, 40(04): 757-762.