芦丁对链脲佐菌素诱导的糖尿病小鼠肾组织形态表现的影响

doi:10.13481/j.1671-587x.20170413

摘要/Abstract

摘要： 目的：探讨芦丁对链脲佐菌素（STZ）诱导的糖尿病小鼠肾组织形态的影响，阐明芦丁对肾组织损害的治疗作用。方法：雄性昆明小鼠70只，其中12只小鼠作为正常组，其余小鼠腹腔注射62.5mg·kg^-1STZ，每天1次，连续5d，制备1型糖尿病模型。随机选取造模成功的48只小鼠分为模型组、低剂量芦丁组（50 mg·kg^-1）、高剂量芦丁组（100 mg·kg^-1）和厄贝沙坦组（45 mg·kg^-1），每组12只。各组小鼠灌胃相应药物，正常组和模型组小鼠灌胃等体积的羧甲基纤维素钠溶液，每天1次，持续8周。测定各组小鼠体质量和随机血糖。全自动生化分析仪检测小鼠血肌酐（Cre）和血尿氮素（BUN）水平，计算肾指数。分别通过HE染色法、Masson染色法和电镜观察小鼠肾组织形态表现。结果：采用小剂量连续注射STZ造模后，成模率达98%。与正常组比较，给药前各组小鼠体质量比较差异无统计学意义（P>0.05），给药后模型组，低、高剂量芦丁组和厄贝沙坦组小鼠体质量均明显下降（P<0.05）。与模型组比较，不同剂量芦丁组小鼠血糖水平明显降低（P<0.05），Cre和BUN明显降低（P<0.05），高剂量芦丁组肾指数明显降低（P<0.05）。与正常组比较，模型组小鼠肾组织损伤严重，肾小球严重萎缩，肾组织纤维化程度严重，肾小球基底膜弥漫性增厚，足突融合甚至消失；与模型组比较，低、高剂量芦丁组小鼠症状明显改善，高剂量芦丁组小鼠改善效果最为明显。结论：芦丁可改善STZ诱导的糖尿病小鼠肾功能，减轻糖尿病小鼠肾组织损害程度。

关键词: 芦丁, 实验性, 糖尿病, 肾组织形态

Abstract: Objective: To study the influence of rutin in the morphology of renal tissue of the diabetic mice induced by streptozotocin(STZ), and to clarify the effect of rutin on the kidney tissue damage.Methods: Twelve mice of the total 70 Kunming mice were used as normal group, the other were used to estabish type 1 diabetes mouse models by intraperitoneally injected with STZ (62.5 mg·kg^-1), once daily for 5 d. The successfully established model mice were randomly divided into model group,low dose (50mg·kg^-1)of rutin group, high dose (100 mg·kg^-1) of rutin group and irbesartan group (45 mg·kg^-1). The mice in model group and normal group were given the carboxy methyl cellulose(CMC) and the other mice were given drugs by intragastric administration once daily for 8 weeks accordingly. The weight and blood glucose of the each mouse were determined.Full automatic biochemical analyzer was used to detect the levels of serum creatinine (Cre) and blood urine nitrogen (BUN) of the mice, and the kidney index was calculated. The morphology of renal tissue was observed by HE staining, Masson staining and electron microscope.Results: After injection of STZ,the model success rate was up to 98%.Compared with normal group, there was no significant difference in the weight of the mice in other groups before administration(P>0.05).After administration of rutin, the weights of the mice in model group, low dose of rutin group, high dose of rutin group and irbesartan group were significantly decreased (P<0.05). Compared with model group, the levels of blood glucose of the mice in low and high doses of rutin groups were significantly decreased(P<0.05);the levels of Cre and BUN were significantly reduced (P<0.05);the kidney index of the mice in high dose of rutin group was significantly decreased (P<0.05). Compared with normal group,the kidney tissue of the mice in model group was seriously damaged;glomerular was weaked, the kidney tissue fibrosis was serious, glomerular basement membrane was diffusely thickened and foot process was coalesced or overgrow. Compared with model group,the degree of injury of the mice in low and high doses of rutin groups were significantly improved, especially in high dose of rutin group.Conclusion: Rutin can improve the renal function of diabetic mice induced by STZ and reduce the degree of renal tissue damage in the diabetic mice

Key words: renal morphology, rutin, diabetes mellitus,experimental

中图分类号:

R-332

白静, 冀晓茹, 韩婷, 安春娜, 勾向博, 苏鹏, 王妍. 芦丁对链脲佐菌素诱导的糖尿病小鼠肾组织形态表现的影响[J]. 吉林大学学报(医学版), 2017, 43(04): 729-733.

BAI Jing, JI Xiaoru, HAN Ting, AN Chunna, GOU Xiangbo, SU Peng, WANG Yan. Influence of rutin in renal tissue morphology in STZ-induced diabetes mice[J]. Journal of Jilin University Medicine Edition, 2017, 43(04): 729-733.

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