通心络抗家兔主动脉粥样硬化的作用及其机制

J4 ›› 2010, Vol. 36 ›› Issue (1): 119-122.

通心络抗家兔主动脉粥样硬化的作用及其机制

宋子凯¹|曲锐¹|秦玲¹|黄可欣²

1. 吉林大学第一医院心内科| 吉林长春 130021；2. 吉林大学基础医学院组织学与胚胎学教研室,吉林长春 130021

收稿日期:2009-06-16 出版日期:2010-01-28 发布日期:2010-01-28
通讯作者: 秦玲（Tel:0431-84808229，E-mail：qinling@medmail.com.cn） E-mail:qinling@medmail.com.cn
作者简介:宋子凯（1985-）|女|内蒙古通辽市人|在读医学硕士|主要从事动脉粥样硬化的基础及临床研究。
基金资助:
吉林省科技厅科研基金资助课题（20050408）

Anti-atherosclerosis |effect of Tongxinluo |in rabbits |and its mechanism

SONG Zi-Kai¹, QU Rui¹, QIN Ling¹, HUANG Ke-Xin²

1.Department of Cardiology, First Hospital,Jilin University,Changchun 130021,China;2. Department of Histology and Embryology,School of Basica Medical Sciences,Jilin University,Changchun 130021,China

Received:2009-06-16 Online:2010-01-28 Published:2010-01-28

摘要/Abstract

摘要：

目的：观察通心络对主动脉粥样硬化家兔血清和动脉粥样硬化（AS）局部纤溶酶原激活物抑制物-1（PAI-1）及血管细胞黏附分子-1（VCAM-1）表达的影响，阐明通心络抗AS的可能机制。方法：24只大耳白兔随机分为常规饲料组、胆固醇饲料组和通心络组，饲养14周，检测实验前后血清胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）、PAI-1、VCAM-1水平的变化；处死动物后取主动脉进行病理学检查；采用免疫组织化学方法测定AS局部PAI-1和VCAM-1阳性表达百分数。结果：实验前三组动物血清TC、TG、LDL、PAI-1和VCAM-1水平比较差异无显著性（P>0.05），14周后胆固醇饲料组和通心络组TC、TG、LDL、PAI-1和VCAM-1水平明显高于对照组（P<0.01），通心络组血清TC、TG、LDL、PAI-1和VCAM-1水平明显低于胆固醇饲料组（P<0.01）；免疫组织化学检测胆固醇饲料组和通心络组AS局部PAI-1和VCAM-1的阳性表达百分数明显高于对照组（P<0.01），通心络组PAI-1和VCAM-1的阳性表达较胆固醇饲料组的表达明显减少（P<0.01）。结论：AS的发生伴有PAI-1和VCAM-1的过度表达，抑制PAI-1和VCAM-1的表达可能是通心络抗AS机制之一。

关键词: 通心络；动脉粥样硬化；纤溶酶原激活物抑制物1；血管细胞黏附分子-1

Abstract:

Abstract:Objective To observe the effect of Tongxinluo on the expreeions of plasminogen activator inhibitor type-1 (PAI-1) and vascular cell adhension molecule-1 (VCAM-1) in rabbits with atherosclerosis(AS),and explore the possible mechanism.Methods Twenty four rabbits were randomly divided into control,cholesterol diet,and Tongxinluo groups.The levels of serum cholesterol(TC),triacylglycerol(TG),low density lipoprotein(LDL),PAI-1 and VCAM-1 were measured before and after treatment.The aortas were harvested for pathologic morphological observation.Immunohistochemistry analysis was used to evaluate the positive percentages of PAI-1 and VCAM-1.Results There were no differences of TC,TG,LDL,PAI-1 and VCAM-1 between three groups before treatment （P>0.05）. 14 weeks after treatment,the TC,TG,LDL,PAI-1 and VCAM-1 levels in cholesterol diet group and Tongxinluo group were significantly higher than those in control group(P<0.01),while the TC,TG,LDL,PAI-1and VCAM-1 levels in Tongxinluo groups were signficantly lower than those in cholesterol diet group(P<0.01). The expressions of PAI-1 and VCAM-1 in aorta in cholesterol diet and Tongxinluo group were higher than those in control group(P<0.01),and the expressions of PAI-1 and VCAM-1 in Tongxinluo group were lower than those in cholesterol group(P<0.01).Conclusion PAI-1and VCAM-1 over express in AS rabbits,while inhibiting the expressions of PAI-1 and VCAM is likely to be one of anti-AS mechanism of Tongxinluo.

Key words: Tongxinluo;atherosclerosis;plasminogen activator inhibitor 1;vascular cell adhesion molecule-1

中图分类号:

R543

宋子凯, 曲锐, 秦玲, 黄可欣. 通心络抗家兔主动脉粥样硬化的作用及其机制[J]. J4, 2010, 36(1): 119-122.

SONG Zi-Kai, QU Rui, QIN Ling, HUANG Ke-Xin. Anti-atherosclerosis |effect of Tongxinluo |in rabbits |and its mechanism[J]. J4, 2010, 36(1): 119-122.

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