吉林大学学报(医学版) ›› 2013, Vol. 39 ›› Issue (3): 441-447.doi: 10.7694/jldxyxb20130304

• 基础研究 • 上一篇    下一篇

不同配穴针刺预防应激性胃溃疡的效应比较及差异表达蛋白的筛选

王朝辉,单纯筱,周丹,严兴科,王富春   

  1. (长春中医药大学针灸推拿学院,吉林  长春  130117)
  • 收稿日期:2012-11-27 出版日期:2013-05-28 发布日期:2013-05-28
  • 通讯作者: 王富春 E-mail:(Tel:0431-86172229,E-mail:fuchenwang420@126.com)
  • 作者简介:王朝辉(1973-),女,吉林省扶余县人,副教授,医学博士,主要从事针灸教学与研究工作。
  • 基金资助:

    国家自然科学基金资助课题(81072863)

Effects of different acupoints of acupuncture on preventing stress ulcer and filtering of differentially expression proteins

WANG Zhao-hui,SHAN Chun-xiao,ZHOU Dan,YAN Xing-ke,WANG Fu-chun   


  1. (College of Acupuncture and Tuina,Changchun University of Chinese Medicine,Changchun 130117,China)
  • Received:2012-11-27 Online:2013-05-28 Published:2013-05-28

摘要: 目的:观察不同配穴的预防性针刺对应激性胃溃疡大鼠胃组织损伤情况及蛋白质谱的影响,阐明不同配穴针刺预防应激性胃溃疡效果,获得相关差异表达蛋白。方法:120只雄性Wistar大鼠随机分为正常对照组、捆绑对照组、应激性胃溃疡模型组、募穴组、下合穴组、合募配穴组、背俞穴组和俞募配穴组,每组15只。各组大鼠针刺后采用水浸-束缚法制备应激性胃溃疡模型,观察各组大鼠胃黏膜损伤形态学表现,计算溃疡指数;提取胃组织蛋白,应用SDS
-聚丙烯酰胺凝胶电泳方法对胃组织匀浆上清液中的蛋白进行梯度分离,采用纳升级电喷雾高效液相仪联合LTQ线性离子阱质谱仪(Eksigent-Thermo LTQ-MS)检测蛋白表达水平。结果:合募配穴组大鼠溃疡指数低于募穴组、下合穴组、背俞穴组和俞募配穴组(P<0.05)。筛选出的感兴趣差异表达蛋白有以下几种:①酶类,包括甘油醛-3-磷酸脱氢酶(GAPDH)、 碳酸酐酶1(Car1)、碳酸酐酶3(Car3)、乌头合酶(Aco2)、α-烯醇化酶(Eno1 )、β烯醇化酶( Eno3 )和γ烯醇化酶;②运动(骨架)蛋白质,包括alpha 1肌动蛋白(Actc1)、β肌动蛋白(Actb)、α肌动蛋白(Acta1)和相对分子质量为43 000的蛋白质 (Actg2);③运输蛋白质,包括血清铁传递蛋白(TF亚型1)和钙网蛋白 (Calr);④热休克蛋白,包括相对分子质量为10 000的热休克蛋白(Hspe1)。结论:合募配穴预防应激性胃溃疡效果优于单穴组及俞募配穴组,机制可能与多种蛋白的表达变化有关。

关键词: 合募配穴, 针刺, 应激性胃溃疡 , 差异表达蛋白

Abstract: To observe the influence of different acupoints of preventive acupuncture on gastric tissue damage of stress ulcer rat and  protein mass spectrometry,and to clarify the effect of different acupoints of preventive acupuncture on preventing stress ulcer,and  to obtain the related diffierential  expression proteins.Methods 120 male Wistar rats were randomly divided into normal control group (n=15),bundled control group (n=15),stress ulcer model group(n=15),Mu point group(n=15),Xiahe point group (n=15),He and Mu points group(n=15),Back-shu group(n=15),and Back-shu and Mu points group(n=15).The stress ulcer model was constructed by water-immersion method after acupuncture.The morphological changes of the damage of the rat gastric mucosa were observed,and the ulcer index was assessed in various groups.The stomach tissue proteins were extracted,and the proteins in supernatant of stomach tissue homogenate were separated  by SDS-PAGE gel electrophoresis method. Nanoliter electrospray mass high-performance liquid  instrument combined with  LTQ linear ion trap mass spectrometer (Eksigent-Thermo LTQ-MS)  was used to detect the expression levels of proteins. Results The ulcer index of the rats in  He and Mu points group was lower than those  in Mu point group, Xiahe point group,Back-shu point  group, Back-shu and Mu points group(P<0.05). After screening,the interesting differential expression proteins were obtained as follows:①Enzymes,including glyceraldehyde 3-phosphate dehydrogenase (GAPDH),carbonic anhydrase 1 (Car1),carbonic anhydrase 3 (Car3),aconitum synthase (Aco2movement),alpha-enolase (of Eno1),beta enolase (Eno3),and gamma enolase;②Skeleton proteins,including alpha1 actin protein (Actc1),beta actin (Actb),α-actin protein (Acta1),and 43 000 protein (Actg2);
③ Transport proteins,including serum transferrin (TF subtype 1) and calreticulin (Calr);④Heat shock protein,including 10 000 heat shock protein (Hspe1).Conclusion The preventive  effect of  He and  Mu points on stress ulcer  is better than those of single point  and Back-shu and Mu points,its mechanism may be associated with a variety of protein expression changes.

Key words: Xiahe and Mu points, acupuncture, stress ulcer, differentially expressed proteins

中图分类号: 

  • R573.1