关键词: 转基因, 疫苗, 逆转录聚合酶链反应

Abstract: Objective To study the effect of microencapsulated tumor cell vaccine against CEA on immune function in tumor-bearing mice. Methods BALB/c mice were inoculated with 1×10⁶ hepatocarcinoma cell line H-22 cells transfected with human CEA encoding sequence. Spleens were removed to prepare single cell suspensions. ConA-induced lymphocyte proliferation and NK cytotoxicity were detected by ³H-TdR incorporation assays. The levels of IL-2 and IFNγ were determined by ELISA and the mRNA expressions of the cytokines were analyzed by RT-PCR. The proportion of T lymphocyte supsets and NK cells were detected by FCM. Results Microencapsulated CEA tumor cell vaccine enhanced mitogen-induced proliferation and NK cytotoxicity of the splenocytes from tumor-bearing mice and promoted the expressions of IL-2 and IFNγ induced with ConA（P<0.05，P<0.01，P<0.05）. Microencapsulated CEA vaccine increased the proportion of CD4 and decreased the proportion of CD8, leading to the increase of CD4/CD8 ratio（P<0.05）. Conclusion Microencapsulated tumor cell vaccine against CEA can promote the immune function in tumor-inoculated mice, indicating that it may be an effective therapeutic vaccine to inhibit CEA positive tumors.

Key words: transgenes, vaccines, reverse transcriptase polymerase chain reaction