关键词: 脑血管病；HLA-DRB1；单核苷酸多态性

Abstract:

Abstract:Objective To evaluate the association between HLA-DRB1 single nucleotide polymorphisms (SNPs)  and cerebrovascular disease in the Han population from North China,and to lay a foundation for immunogenetic research on cerebrovascular disease.Methods 160 patients with cerebrovascular disease and 116 controls were enrolled in the case-control study.According to the principle of informed consent,the indexes of height,weight,waist circumference (WC),abdominal circumference (AC) and body mass index (BMI) were measured;and the venous blood was drawn for the analysis of albumin (ALB),hemoglobin (Hb),blood creatinine (Cr),blood uric acid (UA),fasting plasma glucose (FBG),total cholesterol (TC),triglycerides (TG),high-density lipoprotein (HDL) and low-density lipoprotein (LDL) and DNA extraction.The SNPs of rs2308765,rs3135388 and rs9269186 in HLA-DRB1 were detected by PCR-LDR assay.The goodness-of-fit χ2 test was used for Hardy-Weinberg equilibrium test in case and control groups.The differences of genotypic frequency and allelic frequency between case and control were test by χ2 test.Results There were no significant differences of WC,ALB,Hb,FBG,TC and TG between two groups (P>0.05);Compared with control group,the BMI,AC,Cr and UA in case group were higher than that in control group (P<0.05),while HDL was lower (P<0.05).There were no significant differences of distributions of genotypes and alleles of rs2308765,rs3135388 and rs9269186 sites between two groups.Conclusion There is not a certain correlation between rs2308765,rs3135388,and rs9269186 SNPs  and cerebrovascular disease in Han population from North China.

Key words: cerebrovascular disease;human leukocyte antigen DR beta-1;single nucleotide polymorphism