吉林大学学报(医学版)

• 基础研究 • 上一篇    下一篇

心律宁胶囊中齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷浓度HPLC检测方法的建立及评价

王淑琴,崔东滨   

  1. 吉林省中医药科学院,吉林 长春 130012
  • 收稿日期:2013-07-31 出版日期:2014-03-28 发布日期:2014-05-28
  • 通讯作者: 崔东滨 ( Tel:0431-86816873,E-mail:jlcc_cdb@sina.com) E-mail:jlcc_cdb@sina.com
  • 作者简介:王淑琴(1962-),女,吉林省长春市人,主任药师,主要从事中药化学和中药新药开发研究。
  • 基金资助:

    国家科技重大专项资助课题  (2011ZX09401-305-47)

Establishment and evaluation of  HPLC method for detection of concentration  of oleanolic acid-3-O-β-D- pyran glucuronide in Xinlüning capsule 

WANG  Shu-qin,CUI Dong-bin   

  1. Academy of Chinese Medicine Sciences, Jilin Province,Changchun  130012,China
  • Received:2013-07-31 Online:2014-03-28 Published:2014-05-28

摘要:

目的:采用高效液相色谱(HPLC)法测定心律宁胶囊中齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷的浓度,探讨影响心律宁胶囊中齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷浓度测定的各种因素。方法:采用HPLC法测定齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷浓度。色谱柱为HypersilC18柱(4.6 mm×250.0 mm,5.0  μm),流动相为乙腈-1%冰醋酸(56∶44),检测波长为210 nm,速流为1.00 mL?min-1 ,柱温30℃。结果:在本色谱条件下,齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷的分离效果良好,在14.80~26.64  μg内呈良好的线性关系(r=0.999 9),平均加样回收率为97.4%,相对标准偏差(RSD)为1.4%。结论:采用HPLC法测定心律宁胶囊中齐墩果酸-3-O-β-D-吡喃葡萄糖醛酸苷浓度简便、准确、专属性强,HPLC可作为心律宁胶囊浓度测定的质量控制方法。

关键词: 心律宁胶囊, 齐墩果酸-3-O-&beta, -D-吡喃葡萄糖醛酸苷, 色谱法, 高效液相

Abstract:

Objective  To establish an high performance liquid chromatography(HPLC) method for the determination of oleanolic acid-3-O-β-D- pyran glucuronide concentration in Xinlüning capsule,and to examine the various factors that affect the determination of oleanolic acid-3-O-β-D-pyran glucuronide concentration in Xinlüning capsule.Methods HPLC method was used to detect the concentrantion of oleanolic acid-3-O-β-D-pyran glucuronide.  Chromatogyaphic assay was performed with HypersilC18(4.6 mm×250.0 mm,5.0 μm)column  and acetonitrile-1% glacial acetic acid (56∶44).The column temperature was set at 30℃ and the wavelength was 210 nm and the flow rate  was 1.00 mL?min-1.Results Under this condition,the recorery rate of  oleanolic acid-3-O-β-D- pyran glucuronide   was 97.4% with the relative standard deviation(RSD) of 1.4%.The calibration curve showed good linearity in the range of 14.80-26.64  μg(γ=0.999 9).Conclusion The detection  of  the concentration of oleanolic acid-3-O-β-D-pyran glucuronide in Xinlüning capsule by HPLC method is simple and accurate,which can be used as the quality control method of Xinglüning capsule.

Key words: Xinlü, ning capsule, oleanolic acid-3-O-&beta, -D-pyran glucuronide, chromatography,high performance liquid

中图分类号: 

  •