吉林大学学报(医学版)

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参命源皂甙人参锭中人参皂苷浓度HPLC检测方法的建立及评价

刘竞研1,尹建元1,张大伟2,杨玉霞1,陈凡波1,孙丹丹1,孙振学3,赵芸浩*,孟勤4   

  1. 1. 吉林大学药学院天然药物化学教研室,吉林 长春 130021;2. 明参生物科技股份有限公司,台湾 竹北 30263;3. 吉林油田总医院药学部,吉林 松原 138000;4. 吉林大学药学院药学实验中心,吉林 长春 130021
  • 收稿日期:2013-08-23 出版日期:2014-03-28 发布日期:2014-05-28
  • 通讯作者: 孟 勤(Tel:0431-85619701,E-mail:mengqin1966@163.com) E-mail:mengqin1966@163.com
  • 作者简介:刘竞研(1990-),女,吉林省四平市人,在读药学硕士,主要从事天然药物分子结构优化及药物质量控制的研究。
  • 基金资助:

     吉林省科技厅科技发展计划医药产业发展专项资金资助课题(YYZX201123-4)

Establishment and evaluation of   HPLC method for determination of  concentrations of ginsenosides in Life-Source Ginsenosides Complex

 LIU Jing-yan1,YIN Jian-yuan1,ZHANG Da-wei2, YANG Yu-xia1, CHEN Fan-bo1,SUN Dan-dan1,SUN Zhen-xue,ZHAO Yun-hao*,MENG Qin   

  1. 1. Department of Natural Pharmaceutical Chemistry,School of Pharmacy,Jilin University,Changchun 130021,China;2. Mingshen Biotech Co. Ltd.,Zhubei  30263,China;3.Department of  Pharmacy,General Hospital of Jilin Oil Field,Songyuan 138000,China;4.Experimental Center of Pharmacy,School of Pharmacy,Jilin University,Changchun 130021,China
  • Received:2013-08-23 Online:2014-03-28 Published:2014-05-28

摘要:

目的:建立测定参命源皂甙人参锭中5种人参皂苷(Rg1、Re、Rb1、Rb2和Rd)浓度的高效液相色谱(HPLC)法,为工业生产的质量控制提供依据。方法:采用HPLC法测定参命源皂甙人参锭中人参皂苷的浓度,检测条件:Alltima C18(250.0 mm × 4.6 mm,5.0  μm)色谱柱,以乙腈为流动相A,0.1%磷酸水溶液为流动相B,梯度洗脱;流速为1.0 mL?min-1;检测波长为203 nm;柱温为40℃。结果:人参皂苷Rg1、Re、Rb1、Rb2和Rd的线性关系良好,相关系数(r)均大于0.999 1,平均回收率为99.5%~102.6%;精密度实验、稳定性实验和重复性实验检测人参皂苷Rg1、Re、Rb1、Rb2和Rd的相对标准差(RSD)分别为0.35% ~ 0.76%、0.74% ~ 1.44%和0.78% ~ 1.47%。结论:HPLC法检测参命源皂甙人参锭中人参皂苷浓度的方法准确、简单可行、重复性好,可以用于参命源皂甙人参锭的质量控制。

关键词: 参命源皂甙人参锭, 人参皂苷, 色谱法, 高效液相

Abstract:

Objective
 To establish an high performance liquid chromatography (HPLC) method for the determination of concentrations of  five active ginsenosides (Rg1,Re,Rb1,Rb2,and Rd)in Life-Source Ginsenosides Complex,and to provide  basis for the quality control of industrial production.Methods HPLC method was used to determine the concentrations of five ginsenosides in Life-Source Ginsenosides Complex.The developed conditions of HPLC were as follows:   Alltima C18 (250.0 mm × 4.6 mm,5.0  μm) column;A gradient elution was applied by mixing mobile phase A and B,which consisted of acetonitrile and  0.1% phosphoric acid,respectively;the flow rate was 1.0 mL?min-1,and the detector wave length was 203 nm and the column temperature was 40℃.Results The calibration curve showed good linearity,and the values of correlation coefficient were higher than 0.999 1 for all the analytes,and the average recovery rates were ranged from 99.5% to 102.6%.The relative standard deviation (RSD) detected by  accuracy,stability and repetability tests of five ginsenosides were 0.35%-0.76%,0.74%-1.44%,and 0.78%-1.47%,respectively.Conclusion  The detection of ginsenosides in Life-Soure Ginsenosides Complex with HPLC method is accurate,easy and reproducible,which can be applied to quality control of the Life-Source Ginsenosides Complex.

Key words: Life-Source Ginsenosides Complex, ginsenoside, chromatography,high performance liquid

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