关键词:  , 膀胱肿瘤, 高迁移率族蛋白A2, 上皮钙依赖黏附蛋白E, 免疫组织化学

Abstract:

Abstract:Objective To investigate the expressions of HMGA2 and E-cadherin in bladder transitional cell carcinoma(BTCC) tissue and their relationship with clinical stage，pathological grade and prognosis.Methods The expressions of HMGA2 and E-cadherin in 49 cases of  BTCC （including 41 cases of non-muscle　invasive bladder cancer,8 cases of muscle invasive bladder cancer;24 cases of low grade bladder cancer,25 cases of high  grade bladder cancer）and 5 specimens of normal bladder tissues were detected by  immunohistochemtry，and their correlations to the clinicopathologic features were analyzed．Results The HMGA2 expression was negative,but the E-cadherin expression was positive in 5 cases of  normal bladder tissue.The positive expression rate of HMGA2 and negative expression rate of E-cadherin in bladder cancer tissue was 41% and 43%,respectively；the positive expression rate of HMGA2 and negative expression rate of E-cadherin in non-muscle invasive bladder cancer and muscle invasive bladder cancer were 34%，75% and 37%,75%,respectively;the positive expression rate of HMGA2 in muscle invasive bladder cancer was apparently higher than that in  non-muscle invasive bladder cancer(P<0.05)；the negative expression rate of E-cadherin in muscle invasive bladder cancer was  apparently higher than that in  non-muscle invasive bladder cancer(P<0.05).The positive expression rate of HMGA2 and negative expression rate of E-cadherin in low grade and high grade of BTCC were 25%，56% and 25%，60%， respectively;the positive expression rate of HMGA2 in high grade of BTCC was apparently higher than that in  low grade(P<0.05)；the negative expression rate of E-cadherin in high grade of BTCC was apparently higher than that in  low grade of BTCC(P<0.05).The correlation between HMGA2 and E-cadherin was statistically significant (P<0.05).42 patients were followed up for 18-40 months,among which,18 reccured.The positive expression rate of HMGA2 and negative expression rate of E-cadherin in recurrence and non-recurrence groups were 56%，25% and 67%，33%,respectively;the positive expression rate of HMGA2 in recurrence group was apparently higher than that in  non-recurrence group(P<0.05)；the negative expression rate of E-cadherin in recurrence group was apparently higher than that in non-recurrence group(P<0.05).Conclusion The abnormal expressions of HMGA2 and E-cadherin are related to the malignant tendency of BTCC,and they may be used as markers to predict clinical stage，pathological grade and prognosis of BTCC.

Key words: uring bladder neoplasms, high mobility group protein A2, E-cadherin, immunohistochemistry