抑制ClC-3表达对顺铂诱导人卵巢癌SKOV3/DDP细胞凋亡的促进作用

J4 ›› 2012, Vol. 38 ›› Issue (6): 1106-1109.

抑制ClC-3表达对顺铂诱导人卵巢癌SKOV3/DDP细胞凋亡的促进作用

于春艳¹|韩命龙²|钟加滕³|孙连坤³|刘玉和¹

1.北华大学基础医学院病理学教研室|吉林吉林132013；2.吉林市公安局昌邑分局法医室|吉林吉林132001；3.吉林大学白求恩医学院病理生理学教研室|吉林长春 130021）

收稿日期:2012-06-19 发布日期:2012-11-28
通讯作者: 刘玉和(Tel:0432-64608556，E-mail: bhlyh1959@163.com) E-mail:bhlyh1959@163.com
作者简介:于春艳(1975-),女,吉林省吉林市人|副教授|医学博士|主要从事肿瘤病理学方面的研究。
基金资助:
吉林省教育厅资助课题（吉教科合字2009第156号，吉教科合字2011第117号，吉教科合字2012第394号吉林省科技厅自然科学基金资助课题（201215103）


Promoting effect of inhibiting |ClC-3 expression on apoptosis induced by cisplatin in human ovarian cancer SKOV3/DDP cells

YU Chun-yan¹,HAN Ming-long²,ZHONG Jia-teng³,SUN Lian-kun³,LIU Yu-he ¹

1.Department of Pathology,College of Basic Medicine,Beihua University,Jinlin 132013,China; 2. Department of Firensic Medicine,Jilin Public Security Bureau Changyi Branch,Jinlin 132001,China|3. Department of Pathophysiology,Norman Bethune College of Medicine,Jilin University,Changchun 130021,China

Received:2012-06-19 Published:2012-11-28

摘要/Abstract

摘要：

目的：探讨抑制氯离子通道-3(ClC-3)基因表达对顺铂(DDP)诱导的人卵巢癌SKOV3/DDP细胞凋亡的促进作用，为卵巢癌的治疗提供实验依据。方法：转染pSH1-siRNA-ClC-3 重组质粒至人卵巢癌SKOV3/DDP细胞，Western blotting方法检测转染重组质粒对ClC-3蛋白表达的影响；MTT法检测细胞增殖率；Western blotting方法检测凋亡相关蛋白细胞色素C（Cyt-c）及Caspase-3活化片段蛋白表达。结果：与SKOV3细胞比较，SKOV3/ DDP细胞中ClC-3蛋白表达水平增加( P<0.05)；转染pSH1-siRNA-ClC-3 重组质粒后，SKOV3/DDP细胞ClC蛋白表达水平明显降低；ClC-3-siRNA联合DDP作用后，SKOV3/DDP细胞增殖率下降( P<0.05)，Cyt-c和Caspase-3活化片段蛋白表达水平增加( P<0.05) 。结论：pSH1-siRNA-ClC-3可有效抑制SKOV3/DDP细胞ClC-3蛋白的表达，并促进DDP诱导SKOV3/DDP细胞凋亡。

关键词: 卵巢肿瘤；氯通道-3；顺铂；细胞凋亡

Abstract:

Objective To observe the promoting effect of inhibiting chloride channel-3(ClC-3) gene expression on the apoptosis of human ovarian cancer SKOV3/DDP cells induced by cisplatin (DDP),and to provide the experimental basis for treatment of ovarian cancer.Methods The recombinant plamid pSH1-siRNA-ClC-3 was transfected into SKOV3/DDP cells.The ClC-3 expression changes in SKOV3/DDP cells transfected with pSH1-SiRNA-ClC-3 was detected by Western blotting.The vitality of SKOV3/DDP cells was measured by MTT assay.The expression levels of apoptosis-related proteins cytochrome C (Cyt-C) and Cleaved Caspase-3 were determined byWestern blotting. Results Compared with SKOV3 cells,the ClC-3 expression levels in SKOV3/DDP cells was increased (P<0.05).Transfection of pSH1-SiRNA-ClC-3 suppressed the expression of ClC-3 efficiently in SKOV3/DDP cells (P<0.05).The proliferation rate of SKOV3/DDP cells was decreased (P<0.05),and the cyt-C and Cleaved Caspase-3 expression were increased after treated with ClC-3-siRNA combined with DDP(P<0.05).Conclusion pSH1-SiRNA-ClC-3 could suppress the expression of ClC-3 efficiently and enhance the apoptosis induced by DDP in SKOV3/DDP cells.

Key words: ovarian tumor, chloride channel-3;cisplatin;apoptosis

中图分类号:

R737.31

于春艳|韩命龙|钟加滕|孙连坤|刘玉和. 抑制ClC-3表达对顺铂诱导人卵巢癌SKOV3/DDP细胞凋亡的促进作用[J]. J4, 2012, 38(6): 1106-1109.

YU Chun-yan,HAN Ming-long,ZHONG Jia-teng,SUN Lian-kun,LIU Yu-he. Promoting effect of inhibiting |ClC-3 expression on apoptosis induced by cisplatin in human ovarian cancer SKOV3/DDP cells[J]. J4, 2012, 38(6): 1106-1109.

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