吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (03): 532-537.doi: 10.13481/j.1671-587x.20170314

• 基础研究 • 上一篇    下一篇

黄紫堇总生物碱对β-淀粉样蛋白致老年性痴呆大鼠的保护作用

赵春芳1, 李美娟1, 于维维2, 谢湘林3, 冯琳琳1, 刘宏雁3   

  1. 1. 吉林大学药学院药物分析学教研室, 吉林 长春 130021;
    2. 吉林大学口腔医院急诊科, 吉林 长春 130021;
    3. 吉林大学药学院药理学教研室, 吉林 长春 130021
  • 收稿日期:2016-11-29 出版日期:2017-05-28 发布日期:2017-06-01
  • 通讯作者: 刘宏雁,教授,硕士研究生导师(Tel:0431-85619705,E-mail:hongyl@jlu.edu.cn) E-mail:hongyl@jlu.edu.cn
  • 作者简介:赵春芳(1967-),女,吉林省延吉市人,教授,医学博士,主要从事天然药物活性成分及其质量控制方面的研究。
  • 基金资助:
    吉林省科技厅科技引导计划资助课题(20100908)

Protective effect of total alkaloids of Corydalis Ochotensis on rats with Alzheimer's disease induced by β-amyloid protein

ZHAO Chunfang1, LI Meijuan1, YU Weiwei2, XIE Xianglin3, FENG Linlin1, LIU Hongyan3   

  1. 1. Department of Pharmacoanalysis, School of Pharmacy, Jilin University, Changchun 130021, China;
    2. Department of Emergency, School of Stomatology, Jilin University, Changchun 130021, China;
    3. Department of Pharmacology, School of Pharmacy, Jilin University, Changchun 130021, China
  • Received:2016-11-29 Online:2017-05-28 Published:2017-06-01

摘要: 目的:探讨黄紫堇总生物碱(TAOCO)对β-淀粉样蛋白25-35(Aβ25-35)所致老年性痴呆(AD)大鼠行为学及脑组织病理形态表现的影响,阐明其对AD大鼠的保护作用。方法:Wistar大鼠分为正常对照组(0.5 mL·100g-1蒸馏水,n=9)、模型组(0.5mL·100g-1蒸馏水,n=9)、阳性药组(1.75mg·kg-1盐酸多奈哌齐,n=9)和低、中及高剂量(2.0、4.0、8.0 mg·kg-1) TAOCO组(n=8,n=9,n=9)。大鼠海马定位注射Aβ25-35建立AD模型。手术后第14天开始,连续灌胃给药7 d后,Morris水迷宫实验观察各组大鼠空间学习记忆能力,避暗实验观察大鼠被动回避能力,HE染色观察大鼠大脑皮层和海马组织病理形态表现。结果:水迷宫实验,与模型组比较,低剂量TAOCO组大鼠第4~5天到达平台的潜伏期明显缩短(P<0.05);中剂量TAOCO组大鼠第5天达到平台的潜伏期和游程明显缩短(P<0.05),第3天时朝向角缩小(P<0.05);高剂量TAOCO组大鼠第2和5天到达平台的潜伏期明显缩短(P<0.05),第6天时朝向角明显缩小(P<0.01)。与模型组比较,第7天各剂量TAOCO组大鼠在1.5 min内在平台的停留时间、在平台区的停留距离、平台停留时间/总时间和平台停留距离/总路程明显增加(P<0.05);低和高剂量TAOCO组大鼠经过平台的次数和有效区的停留时间明显增加(P<0.05)。避暗实验,与模型组比较,各剂量TAOCO组大鼠第2天的错误潜伏期和错误次数比较差异无统计学意义(P>0.05)。与模型组比较,低和中剂量TAOCO组大鼠皮层和海马组织病理形态表现无明显差异;高剂量TAOCO组大鼠大脑皮层和海马组织的病理损伤明显改善。结论:TAOCO可改善AD大鼠的学习记忆功能,减轻AD大鼠脑组织的病理性损伤。

关键词: &beta, 黄紫堇总生物碱, -淀粉样蛋白, 大鼠, Wistar, 老年性痴呆

Abstract: Objective: To explore the influence of total alkaloids of Corydalis Ochotensis(TAOCO) on the behavior and pathomorphology of brain tissue of the rats with Alzheimer's disease(AD) induced by β-amyloid protein 25-35(Aβ25-35),and to clarify its therapeutic effects on the AD rats. Methods: The Wistar rats were divided into mormal control group(treated with 0.5 mL·100 g-1 distilled water)(n=9),model group(treated with 0.5 mL·100 g-1 distilled water)(n=9),positive drug group(treated with 1.75 mg·kg-1 donepezil hydrochloride)(n=9), and low,middle and high doses (treated with 2.0,4.0 and 8.0 mg·kg-1) of TAOCO groups(n=8,n=9,n=9).The rat AD models were made by injecting Aβ25-35 into hippocampus.On the 14th day after operation,the rats were administered for 7 d.Morris water maze test was used to detect the spatial learning and memory ability of the rats;dark avoidance task was used to observe the passive avoidance ability of the rats; the pathomorphology of the cerebral cortex and hippocampus of the rats were detected. Results: The Morris water maze test results showed that compared with model group,the latency to platform of the rats in low dose of TAOCO group was decreased on the 4th and 5th days(P<0.05); the latency and swimming distance to reach the platform of the rats in middle dose of TAOCO group were decreased on the 5th day(P<0.05),and the starting angle of the rats was reduced on the 3th day(P<0.05); the latencies to reach the platform of the rats in high dose of TAOCO group were decreased on the 2nd and 5th days(P<0.05),and the starting angle of the rats was decreased on the 6th day(P<0.01). Compared with model group,on the 7th day,the time of staying on platform,distance of staying on platform,time of staying on platform/total time,distance of staying on platform/total distance of the rats in different doses of TAOCO groups were all increased significantly(P<0.05) within 1.5 min.Compared with model group,the times of crossing platform and time of staying in effective area of the rats in low and high doses of TAOCO groups were significantly increased(P<0.05).The dark avoidance task results showed that compared with model group,the error latencies and the error times of the rats in different doses of TAOCO groups had no significant differences on the 2nd day(P>0.05).Compared with model group,there was no obvious improvement of the cerebral cortex and hippocampus injury of the rats in low and middle doses of TAOCO groups.In high dose of TAOCO group,the cerebral cortex and hippocampus injury of the rats were significantly improved. Conclusion: TAOCO can improve the learning and memory function of the AD rats and reduce the pathological injury of brain tissue of AD rats.

Key words: amyloid β-protein, total alkaloids of Corydalis Ochotensis, rats,Wistar, Alzheimer's disease

中图分类号: 

  • R749.16