参红补血颗粒对血虚证小鼠肾脏组织中EPOmRNA和骨髓组织中GM-CSFmRNA表达水平的影响

doi:10.13481/j.1671-587x.20170315

吉林大学学报(医学版) ›› 2017, Vol. 43 ›› Issue (03): 538-542.doi: 10.13481/j.1671-587x.20170315

参红补血颗粒对血虚证小鼠肾脏组织中EPOmRNA和骨髓组织中GM-CSFmRNA表达水平的影响

刘文斌¹, 徐岩², 李智萌², 周佳², 黄晓巍²

1. 吉林省食品药品认证中心药品生产管理科, 吉林长春 130062;
2. 长春中医药大学中医药与生物工程研发中心, 吉林长春 130117

收稿日期:2017-01-10 出版日期:2017-05-28 发布日期:2017-06-01
通讯作者: 黄晓巍,主任药师,硕士研究生导师(Tel:0431-86172082,E-mail:15948000740@163.com) E-mail:15948000740@163.com
作者简介:刘文斌(1963-),男,吉林省长春市人,助理研究员,主要从事药品生产企业GMP认证方面的研究。
基金资助:
吉林省科技厅科技发展计划项目资助课题（20150311015YY）

Effects of Shen Hong Bu Xue Granule on expressions of EPO mRNA in kidney tissue and GM-CSF mRNA in bone marrow tissue in mice with blood deficiency

LIU Wenbin¹, XU Yan², LI Zhimeng², ZHOU Jia², HUANG Xiaowei²

1. Department of Production and Management, Jilin Certification Center for Food and Drug, Changchun 130062, China;
2. Center of Traditional Chinese Medicine and Bioengineering, Changchun University of Traditional Chinese Medicine, Changchun 130117, China

Received:2017-01-10 Online:2017-05-28 Published:2017-06-01

摘要/Abstract

摘要： 目的：观察参红补血颗粒（SHBXG）对血虚证模型小鼠肾组织中促红细胞生成素（EPO）mRNA和骨髓组织中粒细胞集落刺激因子（GM-CSF）mRNA表达水平的影响，探讨SHBXG对血虚证小鼠的保护作用及其机制。方法：乙酰苯肼（APH）和环磷酰胺（CTX）联合应用复制血虚证小鼠模型。60只小鼠分为空白对照组，模型组，复方阿胶浆组，低、中和高剂量SHBXG组；每组10只。连续给药14 d后，收集小鼠血清、肾脏及骨髓组织。血液分析仪检测各组小鼠血液中红细胞（RBC）、血红蛋白（HGB）、红细胞比容（HCT）、白细胞（WBC）及血小板（PLT）水平，ELISA法检测各组小鼠血清中EPO和GM-CSF水平，RT-PCR法检测各组小鼠肾脏组织中EPOmRNA及骨髓组织中GM-CSF mRNA的表达水平。结果：血液分析仪检测，与模型组比较，复方阿胶浆组和各剂量SHBXG组小鼠血液中RBC、HGB、HCT和PLT水平明显升高（P<0.05或P<0.01），复方阿胶浆组和高剂量SHBXG组小鼠血液中WBC水平明显升高（P<0.05）。ELISA法检测，与模型组比较，复方阿胶浆组、各剂量SHBXG组小鼠血清中EPO和GM-CSF水平明显升高（P<0.05）。RT-PCR法检测，与模型组比较，复方阿胶浆组、中和高剂量SHBXG组小鼠肾组织中EPO mRNA和骨髓组织中GM-CSF mRNA表达水平明显升高（P<0.05或P<0.01）。结论：SHBXG可以改善血虚证小鼠血虚症状，其机制可能与提高小鼠肾组织中EPO mRNA和骨髓组织中GM-CSF mRNA的表达水平有关联。

关键词: 促红细胞生成素, 参红补血颗粒, 粒细胞集落刺激因子, 血虚证

Abstract: Objective: To observe the effects of Shen Hong Bu Xue Granule(SHBXG) on the expression levels of erythropoietin(EPO)mRNA in kidney tissue and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA in bone marrow tissue of the mice with blood deficiency, and to investigate the protective effect of SHBXG on the blood deficiency mice and its mechanism. Methods: The mouse model of blood deficiency was established with acetylphenylhydrazine(APH) and cytoxan(CTX).A total of 60 mice were divided into blank control group,model group,Compound E-jiao Slurry group and low,middle,high doses of SHBXG groups(n=10).The serum,kidney and bone marrow tissues from all the mice were collected after 14 d consecutive administration.The levels of red blood cells(RBC),hemoglobin level(HGB),hematocrit(HCT),leukocytes(WBC),and platelet(PLT) in blood of the mice were detected by automatic blood analyzer;the levels of serum EPO and GM-CSF of the mice in various groups mice were detected by ELISA method;the expression levels of EPO mRNA in kidney tissue and GM-CSF mRNA in bone marrow tissue of the mice were detected by RT-PCR method. Results: The results of automatic blood analyzer showed that the levels of RBC,HGB,HCT,and PLT of the mice in Compound E-jiao Slurry group and different doses of SHBXG groups were increased significantly compared with model group (P<0.05 or P<0.01);the levels of WBC of mice in Compound E-jiao Slurry group and high dose of SHBXG group were increased significantly compared with model group(P<0.05).The ELISA results showed that the levels of serum EPO and GM-CSF of the mice in Compound E-jiao Slurry group and different doses of SHBXG groups were increased significantly compared with model group(P<0.05).The PC-PCR results showed that the expression levels of EPO mRNA in kidney tissue and GM-CSF mRNA in bone marrow tissue of the mice in Compound E-jiao Slurry group and middle and high doses of SHBXG groups were increased significantly compared with model group(P<0.05 or P<0.01). Conclusion: SHBXG could improve the blood deficiency symptom in the mice with blood deficiency,and its mechanism may be related to increasing the expression levels of EPO mRNA in kidney tissue and GM-CSF mRNA in bone marrow tissue of the mice.

Key words: Shen Hong Bu Xue Granule, blood deficiency, erythropoietin, granulocyte-macrophage colony-stimulating factor

中图分类号:

刘文斌, 徐岩, 李智萌, 周佳, 黄晓巍. 参红补血颗粒对血虚证小鼠肾脏组织中EPOmRNA和骨髓组织中GM-CSFmRNA表达水平的影响[J]. 吉林大学学报(医学版), 2017, 43(03): 538-542.

LIU Wenbin, XU Yan, LI Zhimeng, ZHOU Jia, HUANG Xiaowei. Effects of Shen Hong Bu Xue Granule on expressions of EPO mRNA in kidney tissue and GM-CSF mRNA in bone marrow tissue in mice with blood deficiency[J]. Journal of Jilin University Medicine Edition, 2017, 43(03): 538-542.

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参红补血颗粒对血虚证小鼠肾脏组织中EPOmRNA和骨髓组织中GM-CSFmRNA表达水平的影响

Effects of Shen Hong Bu Xue Granule on expressions of EPO mRNA in kidney tissue and GM-CSF mRNA in bone marrow tissue in mice with blood deficiency

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