关键词: 人参单体皂苷Rh_2<, sub>, 前列腺肿瘤, 细胞周期

Abstract: To study the mechanism of ginsenoside Rh₂ resistingRM-1 prostatic cancer cells in vitro. Methods After the RM-1 cells were treated with different doses of Rh₂,the cell viability was examined by MTT test.The techniques of morphology and flow cytometry were used to analyze apoptotic cycle of RM-1 cells and calculate apoptotic index.Results After RM-1 cells were treated with Rh₂,the growth of cell line was inhibited. When the doses of Rh₂ were 5,15,25, and 30 mg•L^-1,the apoptotic indexes were 12.0%,36.1%,51.2%， and 69.3%, respectively.The apoptotic rates were 41.9% and 48.9% when the doses of Rh₂ were 15 and 25 mg•L^-1.The RM-1 cells treated with Rh₂ showed a typical apoptotic appearance and hypodiploid peak before G₁ phase.Apoptotic index increased in time- and dose-dependent manner. Conclusion Ginsenoside Rh₂ can induce the apoptosis of RM-1 prostatic cancer cells and has significant anti-tumor effect.

Key words: ginsenoside Rh_2<, sub>, prostatic neoplasms, cell cycle