关键词: 胡桃醌, 宫颈肿瘤, SiHa细胞, 细胞周期

Abstract:

Objective To explore the effects of Juglone on the proliferation and cell cycle of human cervical cancer SiHa cells, and to clarify the therapeutic effect of Juglone in cervical cancer. Methods The SiHa cells at logarithmic phase were divided into control group and different doses (10,20,40,60,80,100 μmol·L^-1) of Juglone groups.The morphological changes of the SiHa cells were observed under inverted phase contrast microscope.The proliferation activities and the inhibitory rats of growth of SiHa cells were measured by MTT assay.The cell cycle of SiHa cells was detected by flow cytometry (FMC). Results Compared with control group,the volume of SiHa cells reduced,the connection with the surrounding cells disappeared,and they detached from the surrounding cells in Juglone groups.Compared with control group,the proliferation activities of the SiHa cells in Juglone groups were decreased (P<0.05) and the inhibitory rates of growth of SiHa cells were increased in a dose-dependent manner in Juglne groups; the IC₅₀ value of Juglone was 20.7 μmol·L^-1.The results of FMC showed that the ratios of SbuG₁ state in Juglone groups were increased,and the ratios of SiHa cells at G₀/G₁ and S phases were decreased,and the ratios at G₂/M phase were increased previously then were decreased in 10 and 100 μmol·L^-1 Juglone groups compared with control group. Conclusion Juglone could inhibit the proliferation of SiHa cells and cause the increase of the SubG₁ ratio of cell cycle.

Key words: Juglone, cervical neoplasms, SiHa cells, cell cycle