关键词: 结直肠肿瘤, 五味子多糖, 侵袭活性, 细胞凋亡

Abstract:

Objective To investigate the effect of Alkaline S.chinenis polysaccharides(ASPS) on the proliferation and invasion abilities of the human colon cancer HT-29 cells,and to clarify the anti-tumor effect of ASPS.Methods 40 Wistar rates were randomly divided into control group(n=10,given saline),and 100,200,400 mg·kg^-1 ASPS groups(n=10),respectively.The serum of the rats in various groups were gotten.The HT-29 cells were treated with the serum of the rats in various groups. The inhibitory rates of proliferation and invasion abilities were tested by MTT and Transwell assay;the expression levels of bcl-2,bax,and Caspase-3 proteins in the supernatant of the cells of the rats in various groups were determined by Western blotting method.Results Compared with control group,the inhibitory rates of proliferation and invasion abilities of the HT-29 cells in 200 and 400 mg·kg^-1 ASPS groups were increased(P<0.05, P<0.01).The expression level of bcl-2 protein in 400 mg·kg^-1 ASPS group was lower than those in other groups (P<0.05),while the expression levels of bax and Caspase-3 proteins were higher than those in control group(P<0.05, P<0.01).Conclusion ASPS can inhibit the proliferation and invasion abilities of the human colon cancer HT-29 cells,and its mechanism may be related to down-regulating the expression of bcl-2 protein and up-regulating the expressions of bax and Caspase-3 proteins,thus activating the apoptotic pathway of HT-29 cells.

Key words: colon cancer, Alkaline S.chinensis polysaccharides, invasive ability, apoptosis