关键词: 哮喘, 儿童, 肿瘤坏死因子α, 半胱氨酸蛋白酶8

Abstract:

Objective To explore the effects of tumor necrosis factor α(TNF-α) signal pathway related proteins in the serum of the asthma children in the pathogenesis of asthma,and to provide the basis for prevention and treatment of asthma in the children.Methods The serum of normal children(control group,n=31) and asthma children(asthma group,n=33) in clinical remission,chronic persistent and acute exacerbation periods were obtained. The expression levels of TNF-α,cysteine-containing aspirate-specific protease-8(Caspase-8),TNF receptor-associated factors-2 (TRAF-2),reportor interacting protein (RIP) and cysteine-containing aspirate-specific protease-3 (Caspase-3) proteins were detected by ELISA assay.Results The expression levels of TNF-α protein in serum of the asthma children in clinical remission group,chronic persistent group,and acute exacerbation group were increased. Compared with control group,the expression levels of TNF-α, Caspase-8,and Caspase-3 proteins in serum of the asthma children in acute exacerbation group were significantly increased(P<0.05);the expression level of Caspase-3 protein in serum of the asthma children in clinical remission group was significantly decreased(P<0.05);but the expression level of Caspase-3 protein in serum of the asthma children in chronic persistent group was significantly increased(P<0.05).There were no significant differences of the expression levels of TRAF2 and RIP proteins between asthma group and control group(P>0.05).Conclusion TNF-α signal pathway is closely related to the development of asthma in children,and its mechanism may be activating the TNF-α pathway to promote the expression levels of Caspase-3 and Caspase-8 in the cells and to induce asthma.

Key words: asthma, children, tumor necrosis factor-α, cysteine containing aspirate specific protease-8