吉林大学学报(医学版) ›› 2016, Vol. 42 ›› Issue (01): 15-18.doi: 10.13481/j.1671-587x.20160104

• 基础研究 • 上一篇    下一篇

CD147通过Beclin-1抑制前列腺癌PC-3细胞自噬

方芳1, 方青1, 赵良中1, 李强1, 陈爽1, 张铎1, 王立国2   

  1. 1. 吉林医药学院检验学院医学免疫学教研室, 吉林吉林 132013;
    2. 吉林医药学院附属医院泌尿外科, 吉林吉林 132013
  • 收稿日期:2015-03-11 发布日期:2016-01-26
  • 通讯作者: 王立国,主任医师,硕士研究生导师(Tel:0432-64560741,E-mail:urolancet@sina.com) E-mail:urolancet@sina.com
  • 作者简介:方芳(1973-),女,吉林省吉林市人,副教授,医学博士,主要从事CD147在前列腺癌中的作用方面的研究。
  • 基金资助:

    国家自然科学基金资助课题(81202031);吉林省教育厅"十二五"教育技术研究项目资助课题(吉教科合字2012第333号);吉林省科技厅科技发展计划项目资助课题(20130101154JC);吉林省教育厅"十二五"科学技术研究重点项目资助课题(吉教科合字2013第346号)

Iihibition of autophagy of prostate cancer PC-3 cells by CD147 with Beclin-1

FANG Fang1, FANG Qing1, ZHAO Liang-zhong1, LI Qiang1, CHEN Shuang1, ZHANG Duo1, WANG Liguo2   

  1. 1. Department of Immunology, School of Medical Laboratory, Jilin Medical College, Jilin 132013, China;
    2. Department of Urinary Surgery, Affiliated Hospital, Jilin Medical College, Jilin 132013, China
  • Received:2015-03-11 Published:2016-01-26

摘要:

目的: 通过建立饥饿诱导的自噬模型,探讨CD147对前列腺癌PC-3细胞自噬作用的影响,并阐明其作用机制。 方法: 通过饥饿诱导方式建立自噬细胞模型。实验分为阴性对照组和RNAi干扰CD147组(PC-3/shCD147组)。GFP-LC3质粒转染观察细胞自噬斑点形成情况,免疫印迹技术检测自噬蛋白LC3和Beclin-1表达,台盼蓝排斥实验检测细胞死亡率。 结果: 与阴性对照组比较,PC-3/shCD147组GFP-LC3斑点细胞数增加(P<0.01),自噬相关蛋白LC3-Ⅱ(P<0.01)和Beclin-1(P<0.05)表达水平升高;与阴性对照组(22.3%±3.5%)比较,PC-3/shCD147组细胞死亡率(38.4%±3.1%)明显升高(P<0.05)。 结论: 在前列腺癌PC-3细胞中,CD147通过Beclin-1抑制饥饿诱导的自噬过程,减少细胞自噬的发生。

关键词: CD147, 自噬, Beclin-1, 细胞增殖, 前列腺癌

Abstract:

Objective: To establish starvation-induced autophagy model and explore the influence of CD147 in autophagy of prostate cancer PC-3 cells,and to clarify its mechanism.Methods: Starvation method was employed to establish autophagy model. The experiment included negative control group and CD147 shRNA group (PC-3/shCD147). The autophagy spot formation was detected by GFP-LC-3 plasmid transfection,and the LC3-Ⅱ and Beclin-1 protein expressions were determined by Western blotting method. Tryan blue exclusion assay was used to analyze the cell death. Results: Compared with negative control group,the number of GFP-LC3 spot cells in PC-3/shCD147 group was increased (P<0.01),and the expression levels of LC3-Ⅱ and Beclin-1 were also increased (P<0.05 or P<0.01). Meanwhile,the cell death rate in PC-3/shCD147 group (38.4%±3.1%)was increased compared with negative control group (22.3%±3.5%)(P<0.05).Conclusion: CD147 can inhibit the starvation-induced autophagy with Beclin-1,and decrease the autophagy death in prostate cancer PC-3 cells.

Key words: CD147, autophagy, Beclin-1, cell proliferation, PC-3 cells, prostate neoplasms

中图分类号: 

  • R737.25