关键词: 表皮生长因子受体酪氨酸激酶, SKOV-3人卵巢癌细胞株, 抑制作用, 细胞凋亡, caspase-3, 山奈酚, 厄洛替尼

Abstract:

Objective: To study the inhibitory effect of epidermal growth factor receptor tyrosine kinase(EGFR-TPK),and to clarify the mechanism of their synergetic inhibitory effects on the growth of ovarian cancer cells. Methods: The human ovarian cancer SKOV 3 cells were cultured and divided into blank group,kaempferol group and tarceva group. The activities of EGFR-TPK were detected by ELISA method.The invasion abilities of SKOV-3 cell lines were determined by Transwell assay. The caspase-3 activities of SKOV-3 cells were measured. The inhibitory rates of growth of SKOV-3 cells were determined using MTT method. Results: The half inhibitory concentrations (IC₅₀)of kaempferol and tarceva were (52.33±0.32)g·L^-1and (1.75±0.18)g·L^-1,there was no significant differences between them(P>0.05).The inhibitory rates of growth of SKOV-3 cells were increased with the increasing of doses of kaempferol and tarceva,and there were significant differences compared with blank group(P<0.05 or P<0.01). The caspase-3 activities of SKOV-3 cells of in kaempferol and tarceva groups were significantly increased compared with blank group(P<0.05). Conclusion: Kaempferol in combination with tarceva has a synergetic effect on the growth of ovarian cancer SKOV-3 cells.

Key words: kaempferol, tarceva, epidermal growth factor receptor tyrosine kinase, ovarian cancer SKOV-3 cells, inhibitory effect, apoptosis, caspase-3