关键词: 痛风宁胶囊, 腺嘌呤, 乙胺丁醇片, 痛风性肾病

Abstract: Objective:To discuss the influence of Tongfengning Capsule(TFN)in the levels of uric acid(UA),creatinine(Cr),monocyte chemotactic protein-1(MCP-1),tumor necrosis factor-α(TNF-α),xanthine oxidase(XOD) and blood urea nitrogen (BUN) in the serum and the cyclooxygenase-2(COX-2) activity in the kidney homogenate in the gouty nephropathy model mice,and to analyze its improvement effect on the pathological changes of kidney,spleen and thymus tissues in the mice. Methods: The mouse models of gouty nephropathy were set up by using adenine combined with ethambutol.A total of 70 mice were divided into control group,model group,low(200 mg·kg^-1),medium(400 mg·kg^-1)and high(800 mg·kg^-1)doses of TFN groups,allopurinol (50 mg·kg^-1) positive drug group and Tongfengshu Tablets(600 mg·kg^-1) positive drug group (n=10).The levels of serum UA,Cr,MCP-1,TNF-α,XOD,BUN and the COX-2 activity in homoggenate were detected and the histopathological changes of kidney,spleen,and thymus tissues of the mice were observed with HE staining. Results: Compared with control group,the serum levels of UA,Cr,BUN,MCP-1,TNF-α and XOD of the mice in model group were significantly increased (P<0.01),and the COX-2 activity in the homogenate of kidney tissue of the mice was increased obviously (P<0.01).Compared with model group,the serum UA,Cr,BUN,MCP-1,TNF-α and XOD levels of the mice in positive drug groups and high dose of TFN group were significantly decreased (P<0.01),but there was no significant differences in low and medium doses of TFN groups(P>0.05);the activities of COX-2 in homogenate of kidney tissue of the mice in positive drug groups and high doses of TFN group were significantly decreased (P<0.01),but there was no significant differences in the low and medium doses of TFN groups(P>0.05).Compared with control group,the glomerular atrophy of the mice was found in model group,a large amount of urate crystals presented in renal tubules and renal interstitium,the boundaries between thymus cortex and medulla were not clear and the cells were sparse;the demarcation between the red and white pulp of spleen in the mice was not clear,the splenic nodules were reduced in different degrees,and congestion occurred in the splenic sinus.Compared with model group,the pathological changes such as renal glomerular atrophy,spleen sinuse congestion and unclear boundaries between thymus cortex and medulla of the mice in postive drug groups and different doses of TFN groups were improved to some extent. Conclusion: TFN has certain effect in improving the organ lesions and the abnormal biochemical indexes caused by gouty nephropathy in the mice.

Key words: gouty nephropathy, adenine, Tongfengning Capsule, Ethambutol Tablets