吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (03): 558-562.doi: 10.13481/j.1671-587x.20180319

• 临床研究 • 上一篇    下一篇

硒蛋白S基因单核苷酸多态性与肝癌发病风险的关联性分析

邢少姬1, 郑连生2, 王殿栋3, 张萱4, 池敏5   

  1. 1. 包头医学院医学技术学院临床生物化学检验与分子诊断学教研室, 内蒙古 包头 014060;
    2. 内蒙古自治区 包头市肿瘤医院胸腹外科, 内蒙古 包头 014030;
    3. 包头医学院第二附属医院肿瘤外科, 内蒙古 包头 014030;
    4. 内蒙古自治区包头市肿瘤医院检验科, 内蒙古 包头 014030;
    5. 内蒙古自治区人民医院核医学科, 内蒙古 呼和浩特 012000
  • 收稿日期:2017-04-04 出版日期:2018-05-28 发布日期:2018-05-31
  • 通讯作者: 邢少姬,副教授(Tel:0472-7167927,E-mail:13664772965@163.com) E-mail:13664772965@163.com
  • 作者简介:邢少姬(1977-),女,内蒙古自治区包头市人,副教授,医学硕士,主要从事临床生物化学检验与分子诊断方面的研究。
  • 基金资助:
    内蒙古自治区科技厅自然科学基金面上项目资助课题(2015MS0812)

Analysis on association between single nucleotide polymorphism of selenoprotein S gene and risk of liver cancer

XING Shaoji1, ZHENG Liansheng2, WANG Diandong3, ZHANG Xuan4, CHI Min5   

  1. 1. Department of Clinical Biochemistry Laboratory and Molecular Diagnostics, Institute of Medical Technology, Baotou Medical College, Baotou 014060, China;
    2. Department of Thoracic and Abdominal Surgery, Baotou Tumor Hospital, Inner Mongolia Autonomous Region, Baotou 014030, China;
    3. Department of Oncological Surgery, Second Affiliated Hospital, Baotou Medical College, Baotou 014030, China;
    4. Department of Clinical Laboratory, Baotou Tumor Hospital, Inner Mongolia Autonomous Region, Baotou 014030, China;
    5. Department of Nuclear Medicine, People's Hospital, Inner Mongolia Autonmous Region, Hohhot 010000, China
  • Received:2017-04-04 Online:2018-05-28 Published:2018-05-31

摘要: 目的:探讨内蒙古自治区汉族人群硒蛋白S(SelS)基因单核苷酸多态性(SNP)在肝癌患者和健康对照者的分布情况,阐明其与肝癌发病风险的关联性。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术检测内蒙古自治区汉族肝癌患者(n=96)和健康对照者(n=135) SelS基因rs34713741位点基因型和等位基因频数分布,结果经测序验证。分析SelS基因SNP与肝癌发病风险的关系。结果:SelS基因rs34713741位点的3种基因型CC、CT、TT及等位基因C、T的频数分布在肝癌组和健康对照组间比较差异均无统计学意义(χ2=2.679,χ2=2.331,P>0.05)。肝癌组和健康对照组饮酒者CC、CT、TT基因型和等位基因C、T频数分布比较差异均有统计学意义(χ2=6.533,χ2=6.417,P<0.05);携带T等位基因的饮酒个体患肝癌的风险增加,是CC基因型的2.297倍(OR=2.297,95% CI:1.202~4.393)。结论:SelS基因和饮酒交互作用可能与内蒙古汉族人群肝癌的发病有关联。

关键词: 基因型, 等位基因, 硒蛋白S, 单核苷酸多态性, 肝肿瘤

Abstract: Objective: To investigate the distribution of single nucleotide polymorphism (SNP) of selenoprotein S(SelS)gene in the patients with liver cancer and the healthy controls,and to clarify the correlation of the SNP and the risk of liver cancer in the Inner Mongolia Han population. Methods: The distribution of genotypic and allelic frequencies at rs34713741 site was detected by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)method among 96 patients with liver cancer and 135 healthy controls,and verified by gene sequencing analysis.The relationship between SNP of SelS gene and the risk of liver cancer was analyzed. Results: There were no differences of the frequencies of CC,CT,and TT genotypes and C and T alleles at rs34713741 site of SelS gene between the patients with liver cancer and the healthy controls(χ2=2.679,χ2=2.331,P>0.05).The differences in the frequencies of CC,CT,and TT genotypes and C and T alleles at rs34713741 site of SelS gene of the drinkers between liver cancer group and healthy control group were significant (χ2=6.533,χ2=6.417,P<0.05).The relative risk of liver cancer in the drinkers carrying T allele was 2.297 times of the drinkers carrying genotype CC(OR=2.297,95%CI:1.202-4.393). Conclusion: The gene-environment interaction of SelS and drinking may associate with liver cancer in the Inner Mongolia Han population.

Key words: allele, selenoprotein S, genotype, liver neoplasms, single nucleotide polymorphism

中图分类号: 

  • R735.7