血管内皮生长因子165b对人肝癌HepG2细胞生物学特性的影响

doi:10.13481/j.1671-587x.20180119

吉林大学学报(医学版) ›› 2018, Vol. 44 ›› Issue (01): 101-105.doi: 10.13481/j.1671-587x.20180119

血管内皮生长因子165b对人肝癌HepG₂细胞生物学特性的影响

赵燕颖¹, 王秀岩², 秦国涛¹, 孙远杰¹, 刘志忠¹, 李然伟²

1. 吉林大学第四医院消化内科, 吉林长春 130011;
2. 吉林大学第二医院泌尿外科, 吉林长春 130041

收稿日期:2017-06-02 出版日期:2018-01-28 发布日期:2018-01-24
通讯作者: 李然伟,主任医师,硕士研究生导师(Tel:0431-85909914,E-mail:zhaoyanying0609@126.com) E-mail:zhaoyanying0609@126.com
作者简介:赵燕颖(1978-),女,吉林省长春市人,副主任医师,医学博士,主要从事消化内科相关疾病的临床诊治和基础方面的研究。
基金资助:
吉林省卫计委青年基金资助课题（2013Q024）；吉林省科技厅自然科学基金资助课题（200905139）

Effect of VEGF165b on biological characteristics of human hepatocellular carcinoma HepG₂ cells

ZHAO Yanying¹, WANG Xiuyan², QIN Guotao¹, SUN Yuanjie¹, LIU Zhizhong¹, LI Ranwei²

1. Department of Gastroenterology, Fourth Hospital, Jilin University, Changchun 130011, China;
2. Department of Urinary Surgery, Second Hospital, Jilin University, Changchun 130041, China

Received:2017-06-02 Online:2018-01-28 Published:2018-01-24

摘要/Abstract

摘要： 目的：探讨血管内皮生长因子165b（VEGF165b）对人肝癌HepG₂细胞生物学特性的影响，并初步研究其作用机制。方法：HepG₂细胞分为空白组（仅加转染试剂）、对照组（转染阴性对照PcDNA3.0表达载体）和PcDNA-VEGF165b组（转染VEGF165b表达载体PcDNA-VEGF165b）。MTT法检测各组HepG₂细胞生存率，RT-PCR法和Western blotting法检测HepG₂细胞中VEGF165b和VEGF165 mRNA和蛋白表达水平，Transwell小室法检测HepG₂细胞迁移能力。结果：与空白组比较，对照组HepG₂细胞中VEGF165b和VEGF165 mRNA和蛋白表达水平无明显变化（P>0.05）。与空白组比较，PcDNA-VEGF165b组细胞中VEGF165b mRNA和蛋白表达水平明显升高（P<0.05），VEGF165 mRNA和蛋白表达水平明显降低（P<0.05）。空白组和对照组细胞生存率比较差异无统计学意义（P>0.05）；与空白组和对照组比较，PcDNA-VEGF165b组细胞生存率有所降低，但差异无统计学意义（P>0.05）。细胞迁移实验，空白组和对照组细胞迁移率比较差异无统计学意义（P<0.05）；与空白组和对照组比较，PcDNA-VEGF165b组HepG₂细胞迁移率明显降低（P<0.05）。结论：VEGF 165b能抑制VEGF165基因和蛋白的表达，VEGF165b对肝癌细胞增殖无明显影响，但可降低肝癌细胞的迁移能力。

关键词: 细胞迁移, 血管内皮细胞生长因子165, 细胞增殖, 血管内皮细胞生长因子165b, 肝肿瘤, HepG₂细胞

Abstract: Objective: To study the effect of vascular endothelial growth factor 165b (VEGF165b) on the biological characteristics of human hepatocellular carcinoma HepG₂ cells,and to explore its mechanism. Methods: The HepG₂ cells were divided into blank group (treated with transfection reagent),control group(transfected with pcDNA3.0 expression vector)and pcDNA-VEGF165b group(transfected with pcNDA-VEGF165b).MTT assay was used to detect the survival rate of HepG₂ cells;RT-PCR and Western blotting method were used to detect the expression levels of VEGF165b and VEGF165 mRNA and protein in the HepG₂ cells;Transwell chamber assay was used to measure the migration ability of HepG₂ cells. Results: Compared with blank group,there was no significant changes in the expression levels of VEGF165b and VEGF165 mRNA and protein in the HepG₂ cells in control group(P>0.05).Compared with blank group,the expression levels of VEGF165b mRNA and protein in the HepG₂ cells in PcDNA-VEGF165b group were significantly increased (P<0.05),and the expression levels of VEGF165 mRNA and protein were significantly decreased(P<0.05).There was no significant difference in the survival rates between blank group and control group (P>0.05).The survival rate of HepG₂ cells in PcDNA-VEGF165b group was lower than those in blank group and control group,but the differences were not statistically significant (P>0.05).The cell migration experiment results showed that there was no significant difference in the migration rate between blank group and control group (P<0.05).Compared with blank group and control group,the migration rate of HepG₂ cells in PcDNA-VEGF165b group was significantly reduced(P<0.05). Conclusion: VEGF165b can inhibit the expressions of VEGF165 mRNA and protein,and VEGF165b has no effect on the proliferation of hepatocellular carcinoma cells; but it can reduce the migration of hepatocellular carcinoma cells.

Key words: liver neoplasms, vascular endothelial growth factor 165, cell migration, HepG₂ cells, vascular endothelial growth factor 165b, cell proliferation

中图分类号:

R735.7

赵燕颖, 王秀岩, 秦国涛, 孙远杰, 刘志忠, 李然伟. 血管内皮生长因子165b对人肝癌HepG₂细胞生物学特性的影响[J]. 吉林大学学报(医学版), 2018, 44(01): 101-105.

ZHAO Yanying, WANG Xiuyan, QIN Guotao, SUN Yuanjie, LIU Zhizhong, LI Ranwei. Effect of VEGF165b on biological characteristics of human hepatocellular carcinoma HepG₂ cells[J]. Journal of Jilin University Medicine Edition, 2018, 44(01): 101-105.

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血管内皮生长因子165b对人肝癌HepG₂细胞生物学特性的影响

Effect of VEGF165b on biological characteristics of human hepatocellular carcinoma HepG₂ cells

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