高压氧对运动疲劳小鼠肝脏组织抗氧化能力和ATP酶代谢的影响

doi:10.13481/j.1671-587x.20180616

摘要/Abstract

摘要： 目的：探讨高压氧（HBO）对运动疲劳小鼠的保护作用，其阐明其作用机制。方法：30只健康雄性昆明小鼠随机分为对照组、疲劳组和HBO干预组（n=10）。对照组小鼠正常活动，每天进行无负重游泳训练；疲劳组和HBO干预组小鼠每天进行负重力竭游泳，每次力竭训练后，疲劳组小鼠放回笼中，HBO干预组小鼠立即置于铺有新鲜钠石灰的HBO舱中。检测各组小鼠力竭游泳时间，ELISA法检测小鼠肝脏组织中丙二醛（MDA）、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）和谷胱甘肽-过氧化物酶（GSH-Px）及总抗氧化能力（T-AOC）水平，电镜观察小鼠肝脏组织形态表现，检测小鼠肝脏组织中ATP酶活性。结果：最后一次力竭训练时，与疲劳组比较，HBO干预组小鼠力竭游泳时间明显延长（P=0.002）。与对照组比较，疲劳组和HBO干预组小鼠肝脏组织中MDA水平升高（P<0.05），而CAT、SOD、GSH-Px和T-AOC水平均降低（P<0.05）；与疲劳组比较，HBO干预组小鼠肝脏组织中MDA水平降低（P<0.05），而CAT、SOD、GSH-Px和T-AOC水平均升高（P<0.05）。对照组小鼠肝脏组织未见明显异常改变；疲劳组小鼠肝脏细胞出现浓缩，内质网增生，线粒体出现肿胀或浓缩，嵴结构发生断裂、变形、融合；HBO干预组小鼠肝脏细胞膜完整，线粒体改变较疲劳组减轻，嵴结构完整，内质网改变亦减轻。与对照组比较，疲劳组和HBO干预组小鼠肝脏组织中Na⁺-K⁺-ATP酶、Ca²⁺-Mg²⁺-ATP酶和总ATP酶活性均降低（P<0.05）；与疲劳组比较，HBO干预组小鼠肝脏组织中Na⁺-K⁺-ATP酶、Ca²⁺-Mg²⁺-ATP酶和总ATP酶活性均升高（P<0.05）。结论：HBO干预可有效延长力竭运动小鼠游泳时间，保护肝脏功能，其机制可能与提高肝脏组织的抗氧化能力及ATP酶活性有关。

关键词: 高压氧, 运动疲劳, 肝脏, 抗氧化能力, ATP酶

Abstract: Objective: To investigate the protective effects of hyperbaric oxygen (HBO) in the exercise-induced fatigue mice, and to elucidate its mechanism.Methods: A total of 30 healthy male Kunming mice were randomly divided into control group, fatigue group and HBO intervention group. The mice in control group accepted normal exercise and swimming training without weight-bearing everyday. The mice in fatigue group and HBO intervention group accepted weight-bearing swimming everyday; after each exhaustive training, the mice in fatigue group were placed in the cage and the mice in HBO intervention group were immediately placed into the hyperbaric chamber with fresh sodium lime. The exhaustive swimming time of the mice in various groups were detected. The levels of malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), glutathione-peroxidase (GSH-Px) and total antioxidant capacity (T-AOC) in liver tissue of the mice in various groups were measured by ELISA method. The morphology of liver tissue of the mice in various groups were observed by electron microscope. The activities of ATPase in liver tissue of the mice in various groups were detected.Results: At the last exhaustive training, the exhaustive swimming time of the mice in HBO intervention group was significantly longer than that in fatigue group (P=0.002). Compared with control group, the levels of MDA in liver tissue of the mice in fatigue group and HBO intervention group were increased (P<0.05); while the levels of CAT, SOD, GSH-Px and T-AOC were decreased (P<0.05). Compared with fatigue group, the level of MDA in liver tissue of the mice in HBO intervention group was decreased (P<0.05), while the levels of CAT, SOD, GSH-Px and T-AOC were increased (P<0.05). There were no obvious abnormal changes in liver tissue of the mice in control group; the livercells of the mice in fatigue group showed concentration and endoplasmic reticulosis, the mitochondria were swollen or concentrated, and the structure of the iliac crest was broken, deformed and fused; but in HBO intervention group, the cytomembrane of liver tissue was intact, the mitochondrial changes were less than that in fatigue group, the structure of fistula was intact, and the changes of endoplasmic reticulum were also alleviated. Compared with control group, the activities of Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase and total ATPase in liver tissue of the mice in fatigue group and HBO intervention group were decreased (P<0.05);compared with fatigue group, the activities of Na⁺-K⁺-ATPase, Ca²⁺-Mg²⁺-ATPase and total ATPase in liver tissue of the mice in HBO intervention group were increased (P<0.05).Conclusion: HBO intervention could effectively prolong the exhaustive swimming time of the mice and protect the function of liver,and its mechanism may be related to improving the antioxidant capacity and ATPase activity of liver tissue.

Key words: hyperbaric oxygen, exercise fatigue, liver, antioxidant capacity, ATPase

中图分类号:

R873

张萍, 张良祥. 高压氧对运动疲劳小鼠肝脏组织抗氧化能力和ATP酶代谢的影响[J]. 吉林大学学报(医学版), 2018, 44(06): 1200-1204.

ZHANG Ping, ZHANG Liangxiang. Effects of hyperbaric oxygen on antioxidant capacity and ATPase metabolism in liver tissue of mice with exercise-induced fatigue[J]. Journal of Jilin University(Medicine Edition), 2018, 44(06): 1200-1204.

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