细丝蛋白A在宫颈癌患者癌组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20190608

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (06): 1238-1242.doi: 10.13481/j.1671-587x.20190608

细丝蛋白A在宫颈癌患者癌组织中的表达及其临床意义

刘秀花¹, 王满¹, 董欣洁², 刘京男¹, 韩薇³, 关英慧¹

1. 吉林大学第一医院二部呼吸科, 吉林长春 130031;
2. 吉林大学第一医院二部病理科, 吉林长春 130031;
3. 吉林大学第一医院肿瘤中心, 吉林长春 130021

收稿日期:2019-01-17 出版日期:2019-12-05 发布日期:2019-12-05
通讯作者: 关英慧,副主任医师,硕士研究生导师(Tel:0431-84808345,E-mail:1186887791@qq.com) E-mail:1186887791@qq.com
作者简介:刘秀花(1983-),女,吉林省长春市人,主治医师,医学硕士,主要从事肿瘤的诊断和治疗方面研究。
基金资助:
国家自然科学基金青年基金资助课题（81702589）；吉林大学第一医院二部科研专项基金资助课题（B16）

Expression of filamin A in cancer tissue of cervical cancer patients and its clinical significance

LIU Xiuhua¹, WANG Man¹, DONG Xinjie², LIU Jingnan¹, HAN Wei³, GUAN Yinghui¹

1. Department of Respiratory Medicine, First Hospital, Jilin University, Changchun 130031, China;
2. Department of Pathology, First Hospital, Jilin University, Changchun 130031, China;
3. Tumor Center, First Hospital, Jilin University, Changchun 130021, China

Received:2019-01-17 Online:2019-12-05 Published:2019-12-05

摘要/Abstract

摘要： 目的：探讨宫颈癌组织中细丝蛋白A （FLNa）的表达及其与宫颈癌患者临床病理学特征的关系，分析FLNa表达与判断预后指标的关联性，阐明FLNa作为评价宫颈癌预后指标的可行性，为临床早期评估宫颈癌预后提供依据。方法：采用免疫组织化学法检测55例宫颈癌组织和癌旁组织中FLNa阳性表达率，RT-PCR法检测20例宫颈癌组织和癌旁组织中FLNa mRNA表达水平，分析FLNa表达与宫颈癌患者临床病理参数的关系。结果：免疫组织化学检测，FLNa定位于细胞质，基底细胞中FLNa表达阳性率高，细胞间质也有部分表达。FLNa在宫颈癌组织中高表达，在癌旁正常组织中不表达。Ⅰ+Ⅱ期宫颈癌患者宫颈癌组织中FLNa阳性表达率低于Ⅲ+Ⅳ期患者（P<0.05）。宫颈癌患者癌组织中FLNa表达与患者TNM分期、淋巴结转移和子宫旁浸润有关联（P<0.05）。RT-PCR检测，在20例宫颈癌组织中FLNa mRNA表达水平高于癌旁正常组织（P<0.05）。结论：FLNa可能成为1项评估宫颈癌患者预后的指标。

关键词: 宫颈肿瘤, 细丝蛋白A, 预后, 免疫组织化学

Abstract: Objective: To investigate the expression of filamin A (FLNa) in cervical cancer tissue and its relationships with the clinicopathological characteristics of the cervical cancer patients, to analyze the correlations between the expression of FLNa and the prognostic indicators, to clarify the feasibility of FLNa as a prognostic indicator for cervical cancer,and to provide the evidence for early clinical evaluation on the prognosis of cervical cancer. Methods: The positive expression rates of FLNa in 55 cases of cervical cancer tissue and adjacent tissue were detected by immunohistochemical method,the expression levels of FLNa mRNA in 20 cases of cervical cancer and adjacent tissues were detected by RT-PCR method, and the relationships between the expression of FLNa and the clinicopathological parameters of the patients with cervical cancer were analyzed. Results: The immunohistochemical results showed that FLNa was localized in the cytoplasm,the positive expression rate of FLNa in the basal cells was high and it expressed partly in the interstitium.FLNa highly expressed in the cervical cancer tissue and did not express in the adjacent normal tissue.The positive expression rate of FLNa in the patients with stage Ⅰ+Ⅱ cervical cancer was lower than that in the patients with stage Ⅲ+Ⅳ cervical cancer (P<0.05).The expression of FLNa in cancer tissue of the patients with cervical cancer was associated with TNM stage,lymph node metastasis and parametrial invasion (P<0.05).The RT-PCR results showed that the expression level of FLNa in 20 cases of cervical cancer tissue was higher than that in adjacent normal tissue (P<0.05). Conclusion: FLNa may become a indicator of evaluation on the prognosis of the patients with cervical cancer.

Key words: cervical cancer, filamin A, prognosis, immunohistochemistry

中图分类号:

R737.33

刘秀花, 王满, 董欣洁, 刘京男, 韩薇, 关英慧. 细丝蛋白A在宫颈癌患者癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2019, 45(06): 1238-1242.

LIU Xiuhua, WANG Man, DONG Xinjie, LIU Jingnan, HAN Wei, GUAN Yinghui. Expression of filamin A in cancer tissue of cervical cancer patients and its clinical significance[J]. Journal of Jilin University(Medicine Edition), 2019, 45(06): 1238-1242.

参考文献

[1] WANG Z,LI C,JIANG M,et al. Filamin A (FLNA) regulates autophagy of bladder carcinoma cell and affects its proliferation,invasion and metastasis[J]. Int Urol Nephrol,2018,50(2):263-273.
[2] VITALI E,BOEMI I,ROSSO L,et al.FLNA is implicated in pulmonary neuroendocrine tumors aggressiveness and progression[J].Oncotarget,2017,8(44):77330-77340.
[3] CHIANG T S,WU H F,LEE F S.ADP-ribosylation factor-like 4C binding to filamin-A modulates filopodium formation and cell migration[J].Mol Biol Cell,2017,28(22):3013-3028.
[4] HU J,LU J,GOYAL A,et al.Opposing FlnA and FlnB interactions regulate RhoA activation in guiding dynamic actin stress fiber formation and cell spreading[J].Hum Mol Genet,2017,26(7):1294-1304.
[5] SHAO Q Q,ZHANG T P,ZHAO W J,et al.Filamin a: insights into its exact role in cancers[J].Pathol Oncol Res,2016,22(2):245-252.
[6] 刘秀花,高航,刘晓亮,等.细丝蛋白A在乳腺癌组织中的表达及其临床意义[J].吉林大学学报:医学版,2012,38(1):102-105.
[7] ZHANG Y,ZHU T,LIU J,et al.FLNa negatively regulated proliferation and metastasis in lung adenocarcinoma A549 cells via suppression of EGFR[J].Acta Biochim Biophys Sin (Shanghai),2018,50(2):164-170.
[8] CAMPOS L S,RODRIGUEZ Y I,LEOPOLDINO A M,et al.Filamin A expression negatively regulates sphingosine-1-phosphate-induced NF-kappaB activation in melanoma cells by inhibition of akt signaling[J].Mol Cell Biol,2015,36(2):320-329.
[9] AI J,HUANG H,LV X,et al.FLNA and PGK1 are two potential markers for progression in hepatocellular carcinoma[J].Cell Physiol Biochem,2011,27(3/4):207-216.
[10] SUN G G,SHENG S H,JING S W,et al.An antiproliferative gene FLNA regulates migration and invasion of gastric carcinoma cell in vitro and its clinical significance[J].Tumour Biol,2014,35(3):2641-2648.
[11] TIAN Z Q,SHI J W,WANG X R,et al.New cancer suppressor gene for colorectal adenocarcinoma: filamin A[J].World J Gastroenterol,2015,21(7):2199-2205.
[12] SUN G G,LU Y F,ZHANG J,et al.Filamin A regulates MMP-9 expression and suppresses prostate cancer cell migration and invasion[J].Tumour Biol,2014,35(4): 3819-3826.
[13] SAVOY R M,GHOSH P M.The dual role of filamin A in cancer: can’t live with (too much of) it,can’t live without it[J].Endocr Relat Cancer,2013,20(6):R341-R356.
[14] JIN Y Z,PEI C Z,WEN L Y.FLNA is a predictor of chemoresistance and poor survival in cervical cancer[J].Biomark Med,2016,10(7):711-719.
[15] TIAN H M,LIU X H,HAN W,et al.Differential expression of filamin A and its clinical significance in breast cancer[J].Oncol Lett,2013,6(3):681-686.
[16] 史建伟,于跃明,王士杰,等.细丝蛋白A抑制人结肠癌SW480细胞体外侵袭转移能力[J].基础医学与临床,2011,31(9): 1000-1005.
[17] HARTWIG J H,STOSSEL T P.Isolation and properties of actin,myosin,and a new actinbinding protein in rabbit alveolar macrophages[J].J Biol Chem,1975,250(14):5696-5705.
[18] ZHOU X,BOREN J,AKYUREK L M.Filamins in cardiovascular development[J].Trends Cardiovas Med,2007,17(7):222-229.
[19] FENG Y,WALSH C A.The many faces of filamin: a versatile molecular scaffold for cell motility and signalling[J].Nat Cell Biol,2004,6(11):1034-1038.
[20] VELKOVA A,CARVALHO M A,JOHNSON J O,et al.Identification of Filamin A as a BRCA1-interacting protein required for efficient DNA repair[J].Cell Cycle,2010,9(7):1421-1433.
[21] GUO Y,LI M,BAI G,et al.Filamin A inhibits tumor progression through regulating BRCA1 expression in human breast cancer[J].Oncol Lett,2018,16(5):6261-6266.
[22] 李小翠,袁博,刘秀花,等.细丝蛋白A对药物诱导的乳腺癌细胞凋亡的影响[J].吉林大学学报:医学版,2012,37(6):1070-1074.
[23] JI Z M,YANG L L,NI J,et al.Silencing filamin A inhibits the invasion and migration of breast cancer cells by up-regulating 14-3-3sigma[J].Current medical science,2018,38(3):461-466.
[24] ZHANG K,ZHU T,GAO D,et al.Filamin A expression correlates with proliferation and invasive properties of human metastatic melanoma tumors: implications for survival in patients[J].J Cancer Res Clin Oncol,2014,140(11):1913-1926.
[25] 杨婧,王延洲,梁志清.组蛋白乙酰化修饰在骨髓间充质干细胞向平滑肌细胞分化中的作用[J].解放军医学杂志,2019,44(5):369-374.
[26] 侯娟妮,杜劲,李秀川,等.线粒体外膜转运蛋白70在高糖高脂诱导的心脏微血管内皮细胞损伤中的作用[J].解放军医学杂志,2018,43(4):283-288.

细丝蛋白A在宫颈癌患者癌组织中的表达及其临床意义

Expression of filamin A in cancer tissue of cervical cancer patients and its clinical significance

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	王闯, 于淑莉, 李桃, 呂蓉蓉, 齐洁敏. 乙酰肝素酶和整合素α5β1蛋白在葡萄胎组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2019, 45(06): 1361-1366.
[2]	吕秀云, 杨婷, 徐磊, 王立红. 重症哮喘患者血清MIP-1α和IL-13水平动态变化及其预后评估价值[J]. 吉林大学学报(医学版), 2019, 45(06): 1401-1407.
[3]	冯秀南, 袁艺, 宿凯笙, 姜振宇. 系统性红斑狼疮患者外周血T淋巴细胞亚群的分布及其与疾病活动和预后的关系[J]. 吉林大学学报(医学版), 2019, 45(06): 1415-1421.
[4]	于秀艳, 万广财, 孙洪帅, 朱华, 高海燕, 吴雪峰. 纤维蛋白原与乳腺癌患者预后关系的Meta分析[J]. 吉林大学学报(医学版), 2019, 45(05): 1092-1097.
[5]	霍小蕾, 裴振, 杨浩, 张毅强, 贾建桃, 韩玲娜. LncRNA-BLACAT1调节CyclinD1/CDKN2B轴对非小细胞肺癌细胞增殖的影响及其机制[J]. 吉林大学学报(医学版), 2019, 45(04): 759-765.
[6]	李婳, 曹艳莎, 赵津平, 任甫, 李宁. 组织芯片技术检测SIRT6蛋白在结肠癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2019, 45(04): 893-898.
[7]	田艳华, 苏凯, 王三春, 童卫芳, 张旭, 魏明雪, 冯青杰. 咽喉部肉瘤样癌5例报告及文献复习[J]. 吉林大学学报(医学版), 2019, 45(03): 697-700.
[8]	常盼盼, 韩帅, 别黎, 李毅平. 轴外型桥小脑角区髓母细胞瘤2例报告及文献复习[J]. 吉林大学学报(医学版), 2019, 45(03): 701-704.
[9]	丁荣波, 张海鹏, 申李胜男, 韩冰. 乳腺癌钙化灶与其临床、病理特征和预后关系的研究进展[J]. 吉林大学学报(医学版), 2019, 45(02): 450-456.
[10]	穆天驰, 刘超, 富帅, 李启阳, 王馨梦, 肖建英. 基础转录激活因子1蛋白在胃癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2019, 45(01): 94-99.
[11]	陈敏, 谢明, 万娟. 癫痫发作患者血清Nesfatin-1动态变化及其对评估病情和短期预后的临床价值[J]. 吉林大学学报(医学版), 2019, 45(01): 105-110.
[12]	卢银亮, 朱永刚, 张宁, 汤玉环, 程光惠. 宫颈癌近距离放射治疗疼痛管理的研究进展[J]. 吉林大学学报(医学版), 2019, 45(01): 211-216.
[13]	李红霞, 宫海叶, 曾萧, 张素素, 刘伯锋. TGF-β1和PLGF在不同类型妊娠滋养细胞疾病患者病变组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1041-1046.
[14]	朱广伟, 郑炜, 黄永建, 华进, 杨树钢, 叶建新. 髓样分化因子88在结直肠癌患者癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1047-1051.
[15]	赵振理, 畅继武, 孙光, 胡少华. AAX14401蛋白在人膀胱癌组织中的表达及其意义[J]. 吉林大学学报(医学版), 2018, 44(05): 1052-1055.