组织芯片技术检测SIRT6蛋白在结肠癌组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20190426

吉林大学学报(医学版) ›› 2019, Vol. 45 ›› Issue (04): 893-898.doi: 10.13481/j.1671-587x.20190426

• 临床研究 • 上一篇

组织芯片技术检测SIRT6蛋白在结肠癌组织中的表达及其临床意义

李婳¹, 曹艳莎¹, 赵津平², 任甫³, 李宁¹

1. 锦州医科大学基础医学院生物化学与分子生物学教研室, 辽宁锦州 121000;
2. 锦州医科大学附属第一医院肿瘤血管与微环境实验室, 辽宁锦州 121000;
3. 锦州医科大学生物人类学研究所, 辽宁锦州 121000

收稿日期:2018-10-29 发布日期:2019-08-02
通讯作者: 李宁,副教授,硕士研究生导师(Tel:0416-4673253,E-mail:157026753@qq.com) E-mail:157026753@qq.com
作者简介:李婳(1990-),女,辽宁省凌海市人,在读医学硕士,主要从事结肠肿瘤基础方面的研究。
基金资助:
辽宁省科技厅科研基金资助课题（201602293）；辽宁省教育厅青年基金资助课题（JYTQN201727）；锦州医科大学生物人类学创新团队开放课题（JYLJ201702）

Expression of SIRT6 protein in colon cancer tissue detected by tissue microarray technique and its clinical significance

LI Hua¹, CAO Yansha¹, ZHAO Jinping², REN Fu³, LI Ning¹

1. Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Jinzhou Medical University, Jinzhou 121000, China;
2. Tumor Angiogenesis and Microenvironment Laboratory, First Affiliated Hospital, Jinzhou Medical University, Jinzhou 121000, China;
3. Biological Anthropology Institute, Jinzhou Medical University, Jinzhou 121000, China

Received:2018-10-29 Published:2019-08-02

摘要/Abstract

摘要： 目的：探讨NAD依赖性蛋白脱乙酰基酶sirtuin-6（SIRT6）蛋白在结肠癌组织中的表达及其与结肠癌患者临床病理参数之间的关系，阐明SIRT6蛋白在结肠癌发生发展中的作用。方法：选取术前均未行放化疗的100例结肠癌患者的结肠癌组织和癌旁组织，采用组织芯片技术和免疫组织化学SP法检测结肠癌组织和癌旁组织中SIRT6蛋白表达水平，采用秩和检验分析SIRT6蛋白表达水平与结肠癌患者临床病理参数的关系，采用Kaplan-Meier生存曲线、单因素和多因素生存分析法分析结肠癌患者临床病理参数与预后的关系。结果：SIRT6蛋白在结肠癌组织细胞核和胞浆中的表达水平均明显高于癌旁组织（Z=-4.603，P=0.000；Z=-7.610，P=0.000）。Kaplan-Meier单因素和多因素分析，细胞核中SIRT6蛋白高表达的结肠癌患者预后更好且是其独立预后影响因子（HR=2.345，P=0.003），年龄较小及T、N分期较低的患者生存率更高（HR=0.394，P=0.004；HR=2.301，P=0.012；HR=2.423，P=0.048），细胞浆中SIRT6蛋白高表达与结肠癌患者预后无关联性（HR=1.309，P=0.333）。结论：SIRT6蛋白在结肠癌细胞核中高表达可作为结肠癌预后良好的评价指标。

关键词: 结肠肿瘤, NAD依赖性蛋白脱乙酰基酶sirtuin-6, 组织芯片, 免疫组织化学

Abstract: Objective:To investigate the expression of NAD-dependent protein deacetylase sirtuin-6(SIRT6) protein in the colon cancer tissue and the association between its expression and the clinicopathological parameters of the patients with colon cancer, and to elucidate the effect of the SIRT6 protein in the occurrence and development of colon cancer. Methods:The colon cancer tissue and the paracancerous tissue obtained from 100 patients who did not receive radiotherapy and chemotherapy before operation were selected.The expression levels SIRT6 protein in colon cancer tissue and paracancerous tissue were detected by tissue microarray technique and immunohistochemical SP method. The relationships between the expression of SIRT6 and the clinicopathological parameters of the patents with colon cancer were analyzed with rank sum test. Kaplan-Meier survival curre,univariate analysis and multivariate survival analysis were used to investigate the relationships between the clinicopathological parameters and the prognosis in the patients with colon cancer. Results:The expression levels of SIRT6 in cytoplasm and nucleus in colon cancer tissue were significantly higher than those in paracancerous tissue (Z=-4.603,P=0.000;Z=-7.610,P=0.000).The Kaplan-Meier univariate and multivariate analysis showed that the prognosis of the patients with high expression of SIRT6 in the nucleus was better, and the expression of SIRT6 was identified as the independent prognostic factor of colon cancer(HR=2.345, P=0.003); the patients with younger age, lower T and N stages had higher survival rates (HR=0.394,P=0.004; HR=2.301,P=0.012; HR=2.423,P=0.048);there was no correlation between the high expression of SIRT6 in cytoplasm and the prognosis (HR=1.309, P=0.333) of the patients with colon cancer. Conclusion:SIRT6 protein is highly expressed in the nucleus of colon cancer and it can be used as a good prognostic indicator for colon cancer.

Key words: colon neoplasms, NAD-dependent protein deacetylase sirtuin-6, tissue microarray, immunohistochemistry

中图分类号:

R735.35

李婳, 曹艳莎, 赵津平, 任甫, 李宁. 组织芯片技术检测SIRT6蛋白在结肠癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2019, 45(04): 893-898.

LI Hua, CAO Yansha, ZHAO Jinping, REN Fu, LI Ning. Expression of SIRT6 protein in colon cancer tissue detected by tissue microarray technique and its clinical significance[J]. Journal of Jilin University(Medicine Edition), 2019, 45(04): 893-898.

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组织芯片技术检测SIRT6蛋白在结肠癌组织中的表达及其临床意义

Expression of SIRT6 protein in colon cancer tissue detected by tissue microarray technique and its clinical significance

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