DNCB对BALB/c小鼠特应性皮炎的诱导作用及其机制

doi:10.13481/j.1671-587x.20200302

吉林大学学报(医学版) ›› 2020, Vol. 46 ›› Issue (03): 439-443.doi: 10.13481/j.1671-587x.20200302

• 基础研究 • 上一篇

DNCB对BALB/c小鼠特应性皮炎的诱导作用及其机制

王欣欣¹, 李思佳¹, 关洪全², 侯殿东²

1. 辽宁中医药大学研究生学院, 辽宁沈阳 110000;
2. 辽宁中医药大学中西医结合学院免疫与病原生物学教研室, 辽宁沈阳 110000

收稿日期:2019-05-22 发布日期:2020-06-11
通讯作者: 关洪全,教授,博士研究生导师(Tel:024-31207156,E-mail:guanhongquan@163.com);侯殿东,副教授,硕士研究生导师(Tel:024-31207095,E-mail:houdiandong@163.com) E-mail:guanhongquan@163.com;houdiandong@163.com
作者简介:王欣欣(1982-),女,内蒙古自治区包头市人,主治医师,讲师,在读医学博士,主要从事天然产物的免疫调节作用机制方面的研究。
基金资助:
国家自然科学基金资助课题（81774023）

Induction effect of DNCB on atopic dermatitis BALB/c mice and its mechanism

WANG Xinxin¹, LI Sijia¹, GUAN Hongquan², HOU Diandong²

1. Graduate School, Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China;
2. Department of Immunology and Pathogenic Biology, College of Integrated Traditional Chinese and Western Medicine, Liaoning University of Traditional Chinese Medicine, Shenyang 110000, China

Received:2019-05-22 Published:2020-06-11

摘要/Abstract

摘要： 目的：采用2，4-二硝基氯苯（DNCB）致敏BALB/c小鼠诱导小鼠发生特应性皮炎（AD），阐明DNCB作为半抗原诱导AD的可能致病机制。方法：12只BALB/c小鼠随机分为对照组和AD模型组，每组6只。于实验第1、4、7天，采用1.0% DNCB溶液涂抹于AD模型组小鼠背部皮肤进行致敏，于实验第14、17、19、22、24、27和29天，采用0.5% DNCB溶液涂抹于AD模型组小鼠左耳背部皮肤进行激发。对照组小鼠于相同时间点外用等体积基质溶液。评价2组小鼠皮肤组织炎症评分，观察2组小鼠耳部皮肤外观变化，HE染色和甲苯胺蓝染色观察2组小鼠左耳皮损部位皮肤组织病理形态表现，测量皮损处皮肤组织上皮层厚度，计数皮损处皮肤组织中肥大细胞数，免疫组织化学染色法检测2组小鼠皮损处皮肤组织中白细胞介素4（IL-4）表达水平；ELISA法检测2组小鼠血清IgE水平。结果：AD模型组小鼠皮肤组织炎症评分明显高于对照组（P<0.01），小鼠左耳部皮肤明显红肿、变硬，伴随出现异常的搔抓行为；AD模型组小鼠皮损处皮肤组织上皮层厚度、浸润肥大细胞数、皮损处皮肤组织中IL-4表达水平和血清IgE水平均明显高于对照组（P<0.05或P<0.01）。结论：外用1.0% DNCB致敏和外用0.5% DNCB激发BALB/c小鼠能够成功诱导AD，其机制可能与二者引起皮肤组织中IL-4表达水平和血清IgE水平升高有关。

关键词: 2,4-二硝基氯苯, 特应性皮炎, BALB/c, 小鼠疾病模型,动物

Abstract: Objective: To induce the atopic dermatitis (AD) by 2,4-dinitrobenzene (DNCB) in the BALB/c mice,and to explore the possible mechanism of DNCB as hapten to induce AD. Methods: A total of 12 BALB/c mice were randomly divided into control group(n=6) and AD model group (n=6). The dorsal skin of the mice in AD model group was sensitized by 1.0% DNCB at days 1,4,and 7 and the back skin of the left ears were challenged by 0.5% DNCB at days 14,17,19,22,24,27,and 29. The mice in control group were given substrate solution with the same volume at the same time points.The inflammation scores of the skin tissue of the mice in two groups were evaluated, the appearance changes of the ear skin of the mice in two groups were observed, the pathomorphology of the skin tissue in left ear of the mice in two groups were observed by HE staining and toluidine blue staining, the epidermal thickness of the skin tissue at lesion site of the mice in two groups was measured,and the number of mast cells in the skin tissue at lesion site of the mice in two groups was counted; the expression levels of interleukin-4(IL-4) in the skin tissue at lesion site of the mice in two groups was detected by immunohistochemistry staining,and the levels of serum IgE was detected by ELISA method. Results: The inflammation score of the mice in AD model group was higher that in control group(P<0.01).The skin tissue in left ear of the mice in AD model presented as redness swelling and hardening accompaning with abnormal scratching marks;the epidermal thickness, the number of mast cells, the expression level of IL-4 in skin tissue at lesion site and the level of serum IgE of the mice in AD model group were all increased compared with control group(P<0.05 or P<0.01). Conclusion: The AD mouse can be successfully induced by 1.0% DNCB for sensitizing and 0.5% DNCB for challenging in the BALB/c mice,and its mechanism may be related with the increasing of IL-4 expression level in skin tissue and IgE level in serum.

Key words: 2,4-dinitrochlorobenzene, atopic dermatitis, BALB/c mice, disease model,animal

中图分类号:

R758.2

王欣欣, 李思佳, 关洪全, 侯殿东. DNCB对BALB/c小鼠特应性皮炎的诱导作用及其机制[J]. 吉林大学学报(医学版), 2020, 46(03): 439-443.

WANG Xinxin, LI Sijia, GUAN Hongquan, HOU Diandong. Induction effect of DNCB on atopic dermatitis BALB/c mice and its mechanism[J]. Journal of Jilin University(Medicine Edition), 2020, 46(03): 439-443.

参考文献

[1] DE VUYST E, SALMON M, EVRARD C, et al. Atopic dermatitis studies through in vitro models[J]. Front Med (Lausanne), 2017, 4:119.
[2] LEUNG D Y, BOGUNIEWICZ M, HOWELL M D,et al. New insights into atopic dermatitis[J]. J Clin Invest, 2004, 113(5):651-657.
[3] PARK K D, PAK S C, PARK K K.The pathogenetic effect of natural and bacterial toxins on atopic dermatitis[J]. Toxins(Basel), 2016, 9(1):E3.
[4] DRUCKER A M, EYERICH K, DEBRUIN-WELLER M S, et al. Use of systemic corticosteroids for atopic dermatitis:International Eczema Council consensus statement[J]. Br J Dermatol, 2018, 178(3):768-775.
[5] SHRESTHA S, MIAO R, WANG L, et al. Burden of atopic dermatitis in the United States:analysis of healthcare claims data in the commercial, medicare, and medical databases[J]. Adv Ther, 2017, 34(8):1989-2006.
[6] ARIMA K, GUPTA S, GADKARI A, et al. Burden of atopic dermatitis in Japanese adults:analysis of data from the 2013 National Health and Wellness Survey[J]. J Dermatol, 2018, 45(4):390-396.
[7] CHOOPANI R, MEHRBANI M, FEKRI A, et al. Treatment of atopic dermatitis from the perspective of traditional persian medicine:presentation of a novel therapeutic approach[J].J Evid Based Complement Altern Med, 2017,22(1):5-11.
[8] YANG J H, LEE E, LEE B, et al. Ethanolic extracts of Artemisia apiacea hance improved atopic dermatitis-like skin lesions in vivo and suppressed TNF-alpha/IFN-gamma(-) induced proinflammatory chemokine production in vitro [J]. Nutrients, 2018, 10(7):E806.
[9] 王俊霞, 杨子微, 车雅敏. 特应性皮炎小鼠模型研究进展[J]. 天津医科大学学报,2017, 23(4):383-385.
[10] KIM J, LEE J, SHIN S, et al. Molecular mechanism of atopic dermatitis induction following sensitization and challenge with 2,4-dinitrochlorobenzene in mouse skin tissue[J]. Toxicol Res, 2018, 34(1):7-12.
[11] HOU D D, DI Z H, QI R Q, et al. Sea buckthorn (Hippophae rhamnoides L.) oilimproves atopic dermatitis-like skin lesions via inhibition of NF-kappaB and STAT1 activation[J]. Skin Pharmacol Physiol, 2017, 30(5):268-276.
[12] 莫俊銮, 周继昌, 刘小立, 等. DNCB致敏诱导BALB/c小鼠特应性皮炎模型的建立[J]. 中国热带医学,2013, 13(12):1439-1442.
[13] MARSELLA R, DE BENEDETTO A. Atopic dermatitis in animals and people:anupdate and comparative review[J]. Vet Sci, 2017, 4(3):E37.
[14] JANG H Y, KOO J H, LEE S M, et al. Atopic dermatitis-like skin lesions aresuppressed in fat-1 transgenic mice through the inhibition of inflammasomes[J]. Exp Mol Med, 2018,50(6):1-9.
[15] SPERGEL J M, MIZOGUCHI E, BREWER J P, et al. Epicutaneous sensitization with protein antigen induces localized allergic dermatitis and hyperresponsiveness to methacholine after single exposure to aerosolized antigen in mice[J]. JClin Invest, 1998,101(8):1614-1622.
[16] 窦侠, 刘小明, 李子卓, 等. 粉尘螨诱导建立"特应性皮炎-哮喘"小鼠模型[J].中国皮肤性病学杂志, 2018, 32(3):275-280.
[17] 李辉, 高强, 王翼腾. 应用小鼠建立特应性皮炎模型的研究进展[J].武警医学, 2015, 26(1):99-102.
[18] YU J S, LEE C J, LEE H S, et al. Prevalence of atopic dermatitis in Korea:analysis by using national statistics[J].J Korean Med Sci, 2012, 27(6):681-685.
[19] SYBILSKI A J, ZALEWSKA M, FURMANCZYK K, et al. The prevalence of sensitization to inhalant allergens in children with atopic dermatitis[J]. Allergy Asthma Proc, 2015, 36(5):e81-e85.
[20] BRANDT E B, SIVAPRASAD U.Th2 cytokines and atopic dermatitis[J]. J Clin Cell Immunol, 2011, 2(3):1-25.
[21] CAMPANA R, DZORO S, MITTERMANN I, et al. Molecular aspects of allergens in atopic dermatitis[J]. Curr Opin Allergy Clin Immunol, 2017, 17(4):269-277.

DNCB对BALB/c小鼠特应性皮炎的诱导作用及其机制

Induction effect of DNCB on atopic dermatitis BALB/c mice and its mechanism

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 4

Metrics

本文评价

推荐阅读 0

[1]	赵东海，张磊，张艳,齐玲,邹向明. 肉苁蓉苯乙醇苷对环磷酰胺致小鼠生精障碍的治疗作用及其机制[J]. 吉林大学学报(医学版), 2014, 40(03): 612-615.
[2]	张怡堃\|李慧\| 黄根山\|董波\|苏振涛. 持续高剂量电磁辐射对小鼠外周血免疫细胞数量影响的长期效应[J]. J4, 2012, 38(5): 856-860.
[3]	徐艳玲, 杜海英, 金明华, 刘晓梅, 张龙, 李鹏, 孙志伟. 氯化镉对裸鼠皮下移植人肝癌SMMC-7721细胞的抑制作用及其对线粒体酶的影响[J]. J4, 2011, 37(1): 56-60.
[4]	盖显英，孙景辉，贺文琦，陆慧君，杜牧，宋德光，陈启军，高丰. 柯萨奇B3病毒川金丝猴分离毒株实验性感染BALB/c小鼠的病理形态学[J]. J4, 2009, 35(1): 39-42.