含缬酪肽蛋白在上皮性卵巢癌组织中的表达及其临床意义

doi:10.13481/j.1671-587x.20200327

摘要/Abstract

摘要： 目的：观察上皮性卵巢癌（EOC）组织中含缬酪肽蛋白（VCP）的表达情况及其与EOC患者临床病理特征的关系，为EOC的分子治疗提供依据。方法：采用免疫组织化学法检测94例EOC组织标本、13例卵巢交界性肿瘤组织标本、36例卵巢良性肿瘤组织标本和8例正常卵巢组织标本中VCP的表达情况。分析VCP阳性表达率与EOC患者各临床病理参数（年龄、FIGO分期、病理类型、组织学分级、是否有淋巴结转移、是否有腹水和术前CA125水平）的关系。采用免疫荧光技术和Western blotting法检测人卵巢上皮HOSEpiC细胞和人EOCSKOV-3细胞中VCP表达；将SKOV-3细胞分为对照组（未经CB-5083处理）和处理组（经CB-5083处理），划痕实验分析2组SKOV-3细胞迁移率，平板克隆形成实验分析2组SKOV-3细胞克隆形成率，免疫荧光法分析2组SKOV-3细胞中VCP表达情况，Western blotting法检测2组SKOV-3细胞中VCP、NF-κB/P65、IκBα和p-IκBα蛋白表达水平。结果：EOC、交界性卵巢肿瘤、良性卵巢肿瘤和正常卵巢组织中VCP阳性表达率组间比较差异有统计学意义（P<0.05）；VCP表达与患者FIGO分期、组织学分级和是否有淋巴结转移有关联（P<0.05），而与患者年龄、病理类型、是否有腹水及术前CA125水平无关联（P>0.05）。SKOV-3细胞中VCP表达水平高于HOSEpiC细胞（P<0.05），处理组SKOV-3细胞迁移率低于对照组（P<0.05），处理组克隆形成率低于对照组（P<0.05），处理组SKOV-3细胞中VCP和NF-κB/P65蛋白表达水平低于对照组（P<0.05），p-IκBα蛋白表达水平高于对照组（P<0.05）。结论：VCP在EOC组织和细胞中呈高表达，VCP高表达可促进肿瘤的迁移和增殖。

关键词: 卵巢肿瘤, 含缬酪肽蛋白, 划痕实验, 平板克隆形成实验

Abstract: Objective: To observe the expression of valosin-containing protein(VCP) in the epithelial ovarian cancer (EOC) tissue and its relationships with the clinicopathological features of the EOC patients,and to provide the basis for the molecular treatment of EOC. Methods: The expressions of VCP in 94 EOC tissue samples,13 ovarian borderline tumor tissue samples,36 ovarian benign tumor tissue samples and 8 normal ovarian tissue samples were measured by immunohistochemical method. The relationships between the VCP expression and the clinicopathological parameters (age,FIGO stage,pathological type,histological grade, lymph node metastasis or not, ascites or not, preoperative CA125 level) of the EOC patients were analyzed.The expressions of VCP in human ovarian epithelial HOSEPIC cells and human EOC SKOV-3 cells were detected by immunofluorescence technique and Western blotting method.The SKOV-3 cells were divided into control group(without CB-5083) and treatment group (with CB-5083), the migration rates of the SKOV-3 cells in two groups were analyzed by scratch experiment, and the clone formation rates of the SKOV-3 cells in two groups were analyzed by plate clone formation experiment. The expressions of VCP in the cells in two groups were analyzed by immunofluorescence technique.The expression levels of VCP, NF-κB/P65,IκBα,and p-IκBα proteins in the SKOV-3 cells in two groups were detected by Western blotting method. Results: The positive expression rates of VCP in EOC, borderline ovarian tumor, benign ovarian tumor and normal ovarian tissues had significant difference (P<0.05);the expression of VCP was associated with the FIGO staging, histological grade, and lymph node metastasis or not(P<0.05), but was not associated with the age, pathological type, ascites or not, and preoperative Ca125 level of the EOC patients (P>0.05).The VCP expression level in the SKOV-3 cells was higher than that in HOSEpiC cells(P<0.05).The migration rate of the SKOV-3 cells in treatment group was lower than that in control group (P<0.05),the cloning formation rate of the SKOV-3 cells in treatment group was lower than that in control group(P<0.05).The expression levels of VCP and NF-κB/P65 proteins of the SKOV-3 cells in treatment group were lower than those in control group (P<0.05),and the expression level of p-IκBα protein was higher than that in control group (P<0.05). Conclusion: VCP is highly expressed in the EOC tissue and cells, and high-expression VCP can promot the tumor migration and proliferation.

Key words: ovarian neoplasm, valosin-containing protein, scratch experiment, plate clone formation experiment

中图分类号:

R737.31

郭小菲, 刘立红, 艾浩. 含缬酪肽蛋白在上皮性卵巢癌组织中的表达及其临床意义[J]. 吉林大学学报(医学版), 2020, 46(03): 600-606.

GUO Xiaofei, LIU Lihong, AI Hao. Expression of valosin-containing protein in epithelial ovarian cancer tissue and its clinical significance[J]. Journal of Jilin University(Medicine Edition), 2020, 46(03): 600-606.

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