针刺单穴和腧穴配伍对胃溃疡大鼠的干预效应及其机制

doi:10.13481/j.1671-587x.20200604

摘要/Abstract

摘要： 目的

探讨单穴和腧穴配伍对胃溃疡大鼠胃黏膜损伤指数、胃黏膜组织形态表现及胃黏膜组织中上皮生长因子受体（EGFR）蛋白表达的干预效应，阐明单穴和腧穴配伍的效应以及针灸治疗大鼠胃溃疡的机制。

方法

100只SD大鼠随机分为空白组、模型组、中脘组、足三里组和合募配伍组，每组20只，除空白组外，其余各组采用束缚-水浸应激法制备应激性胃溃疡大鼠模型，造模成功后，空白组和模型组不予干预，中脘组、足三里组和合募配伍组大鼠分别进行电针中脘、电针足三里、电针合募配伍治疗，10 d后观察各组大鼠胃溃疡情况，检测各组大鼠胃黏膜损伤指数，HE染色观察各组大鼠胃组织病理形态表现，免疫组织化学法检测各组大鼠胃黏膜组织中EGFR蛋白表达水平。

结果

与空白组比较，模型组大鼠胃溃疡指数升高（P<0.05）；与模型组比较，中脘组、足三里组和合募配伍组大鼠胃溃疡指数降低（P<0.05）。HE染色，模型组大鼠胃黏膜被破坏，中脘组、足三里组和合募配伍组大鼠胃黏膜均有不同程度的修复及愈合，以合募配伍组效果最佳。与空白组比较，模型组大鼠胃黏膜组织中EGFR蛋白表达水平升高（P<0.05）；与中脘组比较，足三里组大鼠胃黏膜组织中EGFR蛋白表达水平升高（P<0.05），合募配伍组大鼠胃黏膜组织中EGFR蛋白表达水平升高（P<0.05）；与足三里组比较，合募配伍组大鼠胃黏膜组织中EGFR蛋白表达水平升高（P<0.05）。

结论

电针可通过促进EGFR的表达以治疗胃溃疡，且合募配伍治疗效果最佳，其机制可能与EGFR可以控制胃黏膜细胞增生、分化和生存有关联。

关键词: 腧穴配伍, 胃溃疡, 上皮生长因子受体, 免疫组织化学法, 胃黏膜损伤指数

Abstract: Objective

To investigate the intervention effects of single acupoint and acupoint compatibility on the gastric mucosal injury index， the morphology of gastric mucosa and the expression of epidermal growth factor receptor （EGFR ）protein in gastric tissue of the rats with gastric ulcer， and to clarify the effect of single acupoint and acupoint compatibility and the mechanism of acupuncture for treatment of gastric ulcer in the rats.

Methods

A total of 100 SD rats were randomly divided into blank group， model group， Zusanli group， Zhongwan group， and acupoint compatibility group，and there were 20 rats in each group. Except for blank group， the rats in the other groups were subjected to restraint-water immersion stress method to establish the stress gastric ulcer rat models.After the models were successfully established， no intervention was given to the rats in blank group and model group，and the rats in Zhongwan group， Zusanli group，and acupoint compatibility group were respectively treated with Zhongwan， Zusanli， and Zusanli+Zhongwan electroacupuncture treatment. After 10 d， the gastric ulcer of the rats in various groups was observed， the gastric mucosal injury indexes of the rats in various groups were detected， the pathomorphology of gastric tissue of the rats in various groups was observed by HE staining， and the expression levels of EGFR protein in gastric mucosa tissue of the rats in various groups was detected by immunohistochemistry.

Results

The gastric ulcer index of the rats in model group was higher than that in blank group （P<0.05）， and the gastric ulcer indexes of the rats in Zhongwan group， Zusanli group and acupoint compatibility group were lower than that in model group （P<0.05）. The HE staining results showed that the gastric mucosa of the rats in model group was destroyed，and the gastric mucosa of the rats in Zusanli group， Zhongwan group， and acupoint compatibility group had varying degrees of repair and healing，especially in acupoint compatibility group. The expression level of EGFR protein in gastric mucosa tissue of the rats in model group was higher than that in blank group （P<0.05）；compared with Zhongwan group，the expression level of EGFR protein in gastric mucosa tissue of the rats in Zusanli group was increased （P<0.05），and the expression level of EGFR protein in gastric mucosa tissue of the rats in acupoint compatibility group was increased （P<0.05）； compared with Zusanli group， the expression level of EGFR protein in gastric mucosa tissue of the rats in acupoint compatibility group was increased （P<0.05）.

Conclusion

Electroacupuncture has therapeutic effect on the gastric ulcer by promoting the expression of EGFR， and its mechanism may be related to that EGFR can control the proliferation， differentiation and survival of the gastric mucosal cells.

Key words: compatibility of acupoints, gastric ulcer, epidermal growth factor receptor, immunohistochemical method, gastric mucosal injury index

中图分类号:

R245

哈丽娟,王富春,刘晓娜. 针刺单穴和腧穴配伍对胃溃疡大鼠的干预效应及其机制[J]. 吉林大学学报(医学版), 2020, 46(6): 1131-1136.

Lijuan HA,Fuchun WANG,Xiaona LIU. Intervention effects of single acupoint and acupoint compatibility in gastric ulcer rats and their mechanisms[J]. Journal of Jilin University(Medicine Edition), 2020, 46(6): 1131-1136.

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