关键词: 干细胞, 增殖细胞核抗原, 细胞周期, 细胞凋亡, 蛋白质p53, BCL-2

Abstract: Objective To study the proliferation of hematopoietic cell in patients with myelodysplastic syndrome (MDS). Methods Liquid single layer culture and semisolid culture in vitro were used and flow cytometry was used to detect the cell cycle, apoptosis and the expressions of PCNA, P53,and BCL-2. Results ①The Clony Forming Unit-Granulocyte-Macroplage(CFU-GM) and the Erythroid-Clony Forming Unit(CFU-E) of myelodysplasyic syndromes (MDS/RA) decreased significantly (P<0.001); while CFU-GM,CFU-E of MDS/RAEB could not be detectable; ②The percentage of G1 phase increased and the percentage of S, G₂/M phase decreased significantly (P<0.01), compared with control group; ③The increase of apoptotic bodies of MDS/RA was much more markedly (P<0.01), while the changes of those in MDS/RAEB could not be found significantly; the expression of PCNA of MDS increased in both MDS/RA and MDS/RAEB (P<0.01), especially in MDS/RAEB group;④the protein expression of P53 in MDS increased, while BCL-2 decreased significantly in MDS, compared with normal control (P<0.01). Conclusion There are severe abnormalities in hematopoietic cell of patients with MDS, higher proliferation and excessive apoptosis occur in bone marrow cells and the changes of p53,bcl-2 gene suggest their possible participation in the regulation of apoptosis.

Key words: stem cells, proliferating cell nuclear antigen, cell cycle, apoptosis, protein p53, bcl-2