关键词: 细胞, 培养的, 毛细胞

Abstract: Objective To compare the toxicities between gentamicin and its metabolite, and study the effects of Ca²⁺and Mg²⁺ on the toxicity gentamicin. Methods ①The spiral organ of corti of 10 mice (20 ears) were taken, and divided into 2 groups randomly (n=10), cultured in 0.1 mol·L^-1 gentamicin and the metabolite of 0.1 mol·L^-1 gentamicin for 48h, then the number of hair cells remain was recorded;②The spiral organ of corti of 10 mice were taken (20 ears), and divided into 2 groups randomly (n=10), 3% CaCl₂ and 0.1 mol·L^-1 GM were mixed in the culture medium and the number of hair cells remain in the two medium mentioned above were recorded; ③The spiral organ of corti of 10 mice were taken (20 ears), divided into 2 groups randomly (n=10), 3% MgSO₄ to 0.1 mol·L^-1 GM were mixed in the culture medium, the number of hair cells remain in the two medium mentioned above were recorded. Results It was not significant difference between 0.1 mol·L^-1 GM and the metabolite of 0.1 mol·L^-1 gentamicin (P＞0.05) ; but when 3% CaCl₂ or MgSO₄ were mixed in the culture medium, the number of the survived hair cells was observably increased (P＜0.01). Conclusion The toxicity of GM to the cochlea hair cells has nothing to do with its metabolite; Ca²⁺and Mg²⁺ may act as antagonist to the toxicity of GM in cochlea hair cells.

Key words: cells, cultured, hair cells