尾加压素Ⅱ对大鼠肾小管上皮细胞的促丝裂作用

doi:国家自然科学基金资助课题（30570855）

尾加压素Ⅱ对大鼠肾小管上皮细胞的促丝裂作用

陈素贤，李才，苗春生，张秀云，范哲

（吉林大学再生医学科学研究所病理研究室，吉林长春130021）

收稿日期:2006-06-19 修回日期:1900-01-01 出版日期:2007-03-28 发布日期:2007-03-28
通讯作者: 李才

Mitogenic effect of urotensin Ⅱ on renal tubular epithelial cells in rats

CHEN Su-xian, LI Cai, MIAO Chun-sheng, ZHANG Xiu-yun, FAN Zhe

Department of Pathology, Institute of Frontier Medical Sciences, Jilin University, Changchun 130021, China

Received:2006-06-19 Revised:1900-01-01 Online:2007-03-28 Published:2007-03-28
Contact: LI Cai

摘要/Abstract

摘要： 目的：研究尾加压素Ⅱ（UⅡ）对大鼠肾小管上皮细胞的促丝裂作用。方法：采用机械分离和酶消化法获取肾小管上皮细胞进行培养、鉴定；用免疫细胞化学法定位溴脱氧尿嘧啶核苷（BrdU）掺入到肾小管上皮细胞DNA内；用MTT和BrdU掺入法观察不同浓度UⅡ（0、10^-10、10^-9、10^-8、10^-7和10^-6mol•L^-1）对肾小管上皮细胞增殖和DNA合成的影响，以确定其促丝裂作用。结果：培养的细胞经Cytokeratin-18免疫细胞化学染色后，强度不一和不均匀分布的棕黄色颗粒定位于大部分细胞的胞核周围和胞浆内。培养的肾小管上皮细胞经BrdU掺入和免疫细胞化学显色后，细胞核内有棕黄色颗粒沉着。10^-10 mol •L^-1 UⅡ组的MTT值、 BrdU掺入量与对照组（0 mol•L^-1 UⅡ）比较差异无显著性（P>0.05）；10^-9和10^-8mol•L^-1 UⅡ组的MTT值、BrdU掺入量高于对照组（P<0.01或P<0.05）；与对照组比较，10^-7和10^-6 mol•L^-1 UⅡ组的MTT值明显增加（P<0.05），但增加的幅度较10^-9 和10^-8mol•L^-1 UⅡ组低，而BrdU掺入量无明显改变。结论：UⅡ对体外培养的大鼠肾小管上皮细胞具有促丝裂作用。

关键词: 尾加压素Ⅱ, 肾小管, 上皮细胞 , 促丝裂效应

Abstract: To investigate the mitogenic effects of urotensinⅡ(UⅡ) on renal tubular epithelial cells in rats. Methods The renal tubular epithelialcells in rats were collected by mechanical and enzyme digestive methods and were cultivated and characterized; using immunocytochemistry to investigate bromodeoxyuridine (BrdU) incorporation of DNA in renal tubular epithelial cells of rats; the effects of UⅡ with different concentrations（0,10^-10,10^-9,10^-8,10^-7and 10^-6 mol•L^-1）on proliferation in rat renal tubular epithelial cells were observed using methyl thiazolyl tetrazolium assay (MTT assay) and BrdU incorporation was applied to idetect DNA synthesis in the cells. Results Cytokeratin-18 immunocytochemical staining showed that yellow granules with different intensities and uneven distribution were localized in nucleus surrounding and cytoplasm of the most renal tubular epithelial cells. Yellow granules were deposited in nuclei of cultural renal tubular epithelial cells by immunocytochemical staining following BrdU incorporation. The A values of MTT assay and BrdU incorporation in 10^-10mol•L^-1 UⅡ group were not significantly different from those in control group. In 10^-9and 10^-8 mol•L^-1 UⅡ groups, the A values of MTT assay and BrdU incorporation were significantly higher than those in control group (P<0.01 or P<0.05). Compared with control group, the A values of MTT assay in 10^-7and10^-6 mol•L^-1 UⅡgroups were significantly higher (P<0.05), which was weaker than that difference in 10^-9mol•L^-1 and 10^-8 mol•L^-1 groups, BrdU incorporation were not significantly different. Conclusion UrotensinⅡ reveals a mitogenic effect on rat renal tubular epithelial cells in vitro.

Key words: urotensinⅡ, kidney tubular, epithelial cells, mitogenic effect

中图分类号:

R-332

陈素贤，李才，苗春生，张秀云，范哲. 尾加压素Ⅱ对大鼠肾小管上皮细胞的促丝裂作用[J]. J4, 2007, 33(2): 204-206.

CHEN Su-xian, LI Cai, MIAO Chun-sheng, ZHANG Xiu-yun, FAN Zhe. Mitogenic effect of urotensin Ⅱ on renal tubular epithelial cells in rats[J]. J4, 2007, 33(2): 204-206.

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