关键词: 心肌细胞, H9c2, 细胞凋亡

Abstract: To study the apoptosis of H9c2 myocardial cell lines induced by different concentrations of cadmium chloride(CdCl₂).Methods The H9c2 cells were exposed to 5,10,30,50 and 80 μmol•L^-1 CdCl₂ for 6,12 and 24 h,the survival rate was measured by MTT assay and the apoptotic rate was detected by Annexin Ⅴ-FITC/propidium iodide (PI)-flow cytometry (FCM ) and acridine oringe/ethidium bromide(AO/EB) staining.Results The survival rates in the groups treated with CdCl₂ decreased significantly compared with those in negative controls (P<0.05 or P<0.01). The apoptotic rates in the groups treated with CdCl₂ in creased significantly compared with those of negative controls(P<0.001),but there were no significant differences between various doses groups(P>0.05).The typical morphological changes of early apoptosis in H9c2 cells could be observed under fluorescent microscope by AO/EB staining. Conclusion The H9c2 cells are sensitive to toxicity of CdCl₂,low dose and short time treatment with CdCl₂ can induce apoptosis of H9c2 c

Key words: myocardial cells, H9c2, apoptosis