关键词: 三氯化镧, 肿瘤细胞, 培养的, 肝肿瘤, 细胞周期, P16, P21

Abstract: To study the effects of LaCl₃ on P16 and P21 expressions of hepatocellular carcinoma cells. Methods The experiment was divided into 4 groups.In experiment groups,CBRH-7919 cells were cultivated with 0.01,0.10 and 1.00 mmol•L-1 LaCl₃ in DMEM containing 10％ calf serum.In control group,CBRH-7919 was cultivated in DMEM containing 10％ calf serum without LaCl₃.The growth of CBRH-7919 cells was observed following treated with 0.01,0.10,1.00 mmol•L^-1 LaCl₃ for 1,3,5 d in vitro, respectively,the changes of cell cycle were detected by flow cytometry.At the same time,P16 and P21 were detected by immunocytochemical analysis. Results Compared with control group,the inhibitory rate of growth of CBRH-7919 cells was increased evidently when treated with 0.1 and 1.00 mmol•L^-1 LaCl₃ for 3 and 5 d（P< 0.01）,the percentage of CBRH-7919 cells ofG₀/G₁ was evidently increased（P< 0.01）,the expressions of P16 and P21 were increased（P< 0.01）. Conclusion LaCl₃ can obviously inhibit the growth of CBRH-7919 cells by up-regulating P16 and P21 that can block the cell cycle progress from G₁ to S.

Key words: lanthanum trichloride, tumor cells, cultured, liver neoplasms, cell cycle, P16, P21