20(S)-人参皂苷Rg3与化疗药物联合应用对S180肉瘤的影响

doi:吉林省科技厅科技发展计划资助课题（990575

20(S)-人参皂苷Rg₃与化疗药物联合应用对S180肉瘤的影响

倪劲松，辛颖，王心蕊，石博，陈迪，田阔，吴家祥^*

吉林大学基础医学院病理生物学教育部重点实验室，吉林　长春130021

收稿日期:2005-03-30 修回日期:1900-01-01 出版日期:2005-09-28 发布日期:2005-09-28
通讯作者: 吴家祥

Effect of 20(S)-ginsenoside Rg₃ combinedwith cytotoxic agents on sarcoma 180 of mice

NI Jin-song, XIN Ying,WANG Xin-rui,SHI Bo,CHEN Di,TIAN Kuo,WU Jia-xiang^*

ME Key Laboratory of Pathobiology,School of Basic Medical Sciences, Jilin University, Changchun 130021, China

Received:2005-03-30 Revised:1900-01-01 Online:2005-09-28 Published:2005-09-28
Contact: WU Jia-xiang

摘要/Abstract

摘要： 目的：探讨〖BF〗20(S)-人参皂苷Rg₃（SPG-Rg₃）与化疗药物联合应用对S180肉瘤生长以及荷瘤小鼠生命延长期的影响。方法：体外通过染料排斥实验观察SPG-Rg₃抗肿瘤细胞生长的作用。体内建立S180实体瘤模型，将荷瘤小鼠随机分为6组，即SPG-Rg₃ 3、1和0.3 mg·kg^-1组及环磷酰胺组（CTX）、溶剂对照组（Control）以及联合用药组（CTX+SPG-Rg₃），每日腹腔内给药，持续10 d，停药24 h后处死动物，称体重、瘤重，计算肿瘤生长抑制率（%）及两药相互作用指数（CDI）。并建立S180腹水瘤模型，动物随机分为SPG-Rg₃ 3、1 和0.3 mg·kg^-1组及环磷酰胺组（CTX）、溶剂对照组（Control）以及联合用药组（5-FU+SPG-Rg₃）6组，连续5 d腹腔给药，记录小鼠存活天数，计算生命延长期。结果：体外实验中，SPG-Rg₃抑制了S180肉瘤细胞生长，其IC50值为(10.49±2.96)mg·L^-1。体内实验中，SPG-Rg₃对S180肉瘤生长有明显的抑制作用，（SPG-Rg₃+CTX）联合用药组抑制肿瘤生长的作用优于SPG-Rg₃和CTX组。SPG-Rg₃和5-FU均延长荷瘤小鼠的生存期，（SPG-Rg₃+5-FU）联合用药组生命延长率比5-FU单独用药组明显增高。结论：SPG-Rg₃可明显抑制肿瘤的生长，延长荷瘤小鼠的生存期，且SPG-Rg₃可以提高CTX和5-FU的抗肿瘤作用，有一定的协同增效作用。

关键词: 肉瘤180, 环磷酰胺

Abstract: Objective To study the effects of 20(S)-ginsenoside Rg₃ (SPG-Rg₃) on growth of sarcoma 180 and life of mice with sarcoma 180.Methods In vitro,stain exclusion experiment was used to observe the effect of SPG-Rg₃ on sarcoma 180 cells. In vivo, the mice models of sarcoma 180 were built and divided into 6 groups: 3，1， and 0.3 mg·kg^-1 SPG-Rg₃ groups, CTX group, control group, and SPG-Rg₃ combined with CTX group. Twenty-four hours after inoculating sarcoma cells, mice were administrated by abdominal cavity and killed at the 10th day. The body and neoplasm weights were detected, and the growth suppression rate of tumor and CDI were calculated. The model of ascites sarcoma 180 was also built and CTX was replaced by 5-FU. The method of diviving groups was same as before. Mice were administrated for 5 d and after that, the life of mice with sarcoma 180 was recorded and life elongation term was calculated. Results In vitro, SPG-Rg₃ inhibited the growth of sarcoma 180 cells and the IC⁵⁰ value was (10.49±2.96)mg·L^-1. In vivo, SPG-Rg₃ also inhibited the growth of sarcoma 180 and the inhibitory effect of SPG-Rg₃ combined with CTX was superior to those of SPG-Rg₃ or CTX. SPG-Rg₃ and 5-FU also prolonged life of mice and the rate of life elongation in SPG-Rg₃ combined with 5-FU group was higher than that in 5-FU group. Conclusion SPG-Rg₃ can inhibite the growth of sarcoma 180 and prolong the life of mice with sarcoma 180. Foremore, SPG-Rg₃ can reinforce the inhibitory effects of CTX and 5-FU on tumor growth.

Key words: sarcoma 180, cydophosphamide

中图分类号:

R286

倪劲松，辛颖，王心蕊，石博，陈迪，田阔，吴家祥. 20(S)-人参皂苷Rg₃与化疗药物联合应用对S180肉瘤的影响[J]. J4, 2005, 31(5): 705-708.

NI Jin-song, XIN Ying,WANG Xin-rui,SHI Bo,CHEN Di,TIAN Kuo,WU Jia-xiang. Effect of 20(S)-ginsenoside Rg₃ combinedwith cytotoxic agents on sarcoma 180 of mice[J]. J4, 2005, 31(5): 705-708.

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20(S)-人参皂苷Rg₃与化疗药物联合应用对S180肉瘤的影响

Effect of 20(S)-ginsenoside Rg₃ combinedwith cytotoxic agents on sarcoma 180 of mice

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