红鹿制剂延缓皮肤老化的研究

doi:吉林省科技厅项目资助课题(20001109)

红鹿制剂延缓皮肤老化的研究

郝利铭，姜文华，董智勇，孟晓婷

吉林大学基础医学院组织学与胚胎学教研室，吉林长春130021

收稿日期:2003-05-28 修回日期:1900-01-01 出版日期:2005-09-28 发布日期:2005-09-28

Experimental study on effects of Rhodosinand deer serum preparation on aging skin

HAO Li-ming, JIANG Wen-hua, DONG Zhi-yong，MENG Xiao-ting

Department of Histology and Embryology, Schoolof Basic Medical Sciences, Jilin University, Changchun 130021,China

Received:2003-05-28 Revised:1900-01-01 Online:2005-09-28 Published:2005-09-28

摘要/Abstract

摘要： 目的：研究红鹿制剂对老化皮肤的影响。方法：背部被剪去3 cm×3 cm毛的12只20月龄豚鼠被随机均分为给药组和对照组，给药组涂抹红鹿制剂，对照组涂抹化妆品基质，连续涂抹60 d；应用天狼猩红、HE染色和体视学技术分别观察和测定老化皮肤中Ⅲ型胶原的分布、表皮基底层细胞的分裂指数、角质层同表皮厚度的比例、真皮胶原纤维和微血管的体密度和成纤维细胞的数目。结果：乳头层的Ⅲ型胶原给药组明显多于对照组；基底细胞分裂指数给药组明显多于对照组（P<0.01）；乳头层处微血管的体密度给药组明显大于对照组（P<0.01）；乳头层处成纤维细胞数给药组明显多于对照组（P<0.01），在网状层处给药组明显少于对照组（P<0.01）；角质层同表皮厚度的比例给药组显著低于对照组（P<0.01）；胶原纤维的体密度给药组显著低于对照组（P<0.05）。结论：红鹿制剂能延缓皮肤老化。

关键词: 老化, 抗衰老药(中药), 红鹿制剂

Abstract: Objective To study the effects of Rhodosin and deer serum preparation on aging skin. Methods 12 guinea pigs of 20 months with 3 cm×3 cm hair sheared from their backs were divided into control group and experimental group. Rhodosin and deer serum preparation was besmeared on the skin of guinea pigs in experimental group, and cosmetic matrix was besmeared on the skin of animals in control group for 60 d. The distribution of type Ⅲ collagen was observed with Sirius red staining and HE staining. The fission index of the cells of stratum basale, the thickness proportion of stratum corneum to epidermis, the volume density of the collagen fibers and small blood vessel in dermis, the number of fibroblasts in aging skin were meassured with technique of sterology. Results Compared with the control group, the distribution of type Ⅲ collagen in papillary layer, the fission index of the cells of stratum basale, the number of fibroblasts in papillary layer and the volume density of small blood vessel were increased in the experimental group (P<0.01); the volume density of the collagen fibers in dermis and the thickness proportion of stratum corneum to epidermis were reduced in the experimental group (P<0.01 or P<0.05). Conclusion Rhodosin and deer serum preparation can defer the aging on skin.

Key words: aging, anti-aging drugs (TCD), Rhodosin and deerserum preparation

中图分类号:

R285

郝利铭，姜文华，董智勇，孟晓婷. 红鹿制剂延缓皮肤老化的研究[J]. J4, 2005, 31(5): 709-712.

HAO Li-ming, JIANG Wen-hua, DONG Zhi-yong，MENG Xiao-ting. Experimental study on effects of Rhodosinand deer serum preparation on aging skin[J]. J4, 2005, 31(5): 709-712.

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