关键词: 心肌, 成纤维细胞, 细胞周期蛋白质依赖激酶类, 转化生长因子β

Abstract: To investigate the effect of taurine (Tau) on the myocardial fibrosis and its mechanism. MethodsThe rat model of myocardial fibrosis was built by subcutaneous injection of isoprel(Iso).And the rats were divided into control group,model group and taurine group(80,160,320 mg•kg^-1•d ^-1).Hydroxyproline measurment was carried out for detecting collagen quantification in myocardium tissue.Histopathology changes were detected by HE staining.In vitro,the cultured neonatal rat cardiac fibroblasts(CFb) were divided into control group,model group and taurine group(40，80，160mmol•L^-1).The proliferation of CFb was induced by angiotensin Ⅱ(AngⅡ) and detected by the thiazoleblue (MTT)colorimetric assay.TGF-β₁ level was determined by ELISA technique.The expression of cyclin dependent kinase inhibitor p27 was assessed with flow cytometry. Results In vivo,the contents of hydroxyproline were decreased in Tau(160，320 mg•kg^-1•d^-1) groups as compared with model group(P<0.01 or P<0.001).HE staining results showed that myocardium fiber appeared hyperplastic and were arranged disorder and kytoplasm swelled in model group and Tau relieved the damage of myocardium induced by Iso.CFb proliferation rate and the level of TGF-β₁ were greatly declined when CFb was treated with taurine (40,80 and 160 mmol•L^-1) for 24 h (P<0.05 or P<0.01).The P27 expression was significantly increased in Tau groups（80 and 160mmol•L^-1）as compared with model group(P<0.05 or P<0.001).ConclusionTau can inhibit the proliferation of CFb and metabolism of collagen and offer protection against the myocardial fibrosis by decreasing the level of TGF-β₁ and up-regulating P27 expression.

Key words: myocardium, fibroblasts, cyclin-dependent kinases, transforming growth factor beta