草苁蓉多糖的抗肿瘤作用及免疫调节作用

doi:吉林省科技厅基金资助课题（20050413-2）

草苁蓉多糖的抗肿瘤作用及免疫调节作用

侯元¹，霍德胜²，魏艳君³，迟立超⁴,费瑞⁴

（1．吉林大学基础医学院病原免疫细胞遗传实验中心，吉林长春 130021； 2. 吉林大学基

收稿日期:2007-03-15 修回日期:1900-01-01 出版日期:2007-11-28 发布日期:2007-11-28
通讯作者: 费瑞

Anti-tumor function and immuno-regulatory function of Boschniakia rossica polysaccharide

HOU Yuan¹,HUO De-sheng²,WEI Yan-jun³,CHI Li-chao⁴,FEI Rui⁴

（1. Experimental Center of Pathogenobiology,Immunology,Cell Biology and Genetics,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2. Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;3.Department of Emergency,China-Japan Union Hospital,Jilin University,Changchun 130033,China;4. Department of Cell Biology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China）

Received:2007-03-15 Revised:1900-01-01 Online:2007-11-28 Published:2007-11-28
Contact: FEI Rui

摘要/Abstract

摘要： 目的：观察草苁蓉多糖对荷瘤小鼠免疫器官的调节作用及对免疫功能的影响。方法：接种肝癌细胞H₂₂的荷瘤小鼠随机分为50、100、200和400 mg•kg^-1•d^-1草苁蓉多糖组、阴性对照组及阳性对照组（环磷酰胺组），按剂量给药。10 d后处死小鼠，分别称量脾重、胸腺重，检测荷瘤小鼠脾脏指数和胸腺指数；采用MTT法测定NK细胞活性；淋巴细胞转化实验测定T淋巴细胞转化率(LTT)；中性红比色法观察小鼠腹腔巨噬细胞的吞噬能力；通过称量各小鼠实体瘤的重量，计算抑瘤率。结果：草苁蓉多糖能够显著抑制肿瘤生长，抑瘤率达38.86%;与阴性对照组比较,实验组荷瘤小鼠的脾脏指数显著提高(P<0.01)，胸腺指数则有降低趋势，T淋巴细胞的转化率、NK 细胞的活性、巨噬细胞的吞噬能力显著增强(P<0.01)，与给药剂量存在正相关关系。结论：草苁蓉能抑制肿瘤生长，对小鼠免疫功能具有调节作用。

关键词: 免疫活性, 淋巴细胞转化实验, NK细胞活性, 抑瘤率

Abstract: To study the regulatory function of Boschniakia rossica polysaccharide on immune organ of mice bearing tumor and effect on immunological function.MethodsThe rats inoculated with hepatoma carcinoma cell H₂₂ were divided into six groups at random:50,100,200 and 400 mg• kg^-1•d^-1 Boschniakia rossica polysaccharide groups;negative group and positive group.10 d later,the mice were killed,then the spleens and thymuses were weighed,the effect of Boschniakia rossica polysaccharide on spleens and thymus index were determined.MTT was used to determine the activity of NK cells,lymphocyte transformation test was performed to determine LTT,the phagocytic activity of peritoneal macrophage was observed.The tumor inhibitory rate was calculated. Results Boschniakia rossica polysaccharide depressed the growth of tumor obviously,its tumor inhibitory rate was 38.86%.Compared with negative control group,the spleen indexes of mice bearing tumor in experiment were increased groups(P<0.01)，and thymus index had tendency of decreasing,the transformation efficiency of T lymphocyte,the activity of NK cell and the phagocytic activity of peritoneal macrophage were enhanced(P<0.01),they were positively correlated with medicative dosage.ConclusionBoschniakia rossica polysaccharide can depress the growth of tumor,and has the regulatory effects on immunological function in mice.

Key words: immunity activity, lymphocyte transformation, tumor Inhibition ratio, NK cell activity

中图分类号:

R-332

侯元，霍德胜，魏艳君，迟立超,费瑞. 草苁蓉多糖的抗肿瘤作用及免疫调节作用[J]. J4, 2007, 33(6): 1022-1025.

HOU Yuan,HUO De-sheng,WEI Yan-jun,CHI Li-chao,FEI Rui. Anti-tumor function and immuno-regulatory function of Boschniakia rossica polysaccharide[J]. J4, 2007, 33(6): 1022-1025.