关键词: 心肌, 成纤维细胞, 一氧化氮合酶, 一氧化氮

Abstract: To investigate the effects of angiotensin Ⅰ-converting enzyme inhibitor Enalaprilat （Ena） on proliferation and nitric oxide synthase-nitric oxide system of neonatal rat cardiac fibroblasts （CFb）and to probe its anti-cardiac fibrosis mechanism.MethodsThe cultured neonatal Wistar rat CFb were divided into control group,model group and three doses of Ena groups.CFb were isolated by trypsin digestion method.MTT colorimetric assay was adopted to evaluate cell proliferation,flow cytometry was used to measure cell cycle,nitric acid reductase method and spectrophotometry were used to detect the NO contents and NOS activity respectively with Ena. Results Ena could inhibit CFb proliferation induced by AngⅡ,there were significant differences of MTT values between model group and Ena groups after treated for 24,48 and 72 h(P<0.05 or P<0.01);compared with model group,Ena increased the percentage of cells at phase G₀/G₁,and decreased the percentage of cells at phase S,there were significant differences(P<0.05 or P<0.01);compared with model group,Ena increased the level of NOs/NO of CFb supernatant,the NOS activity and NO content increased significantly with the increasing of dose and time (P<0.05 or P<0.01) . ConclusionThe anti-proliferative effects of Ena on CFb can be attributed to enhancing nitric oxide synthase-nitric oxide system of cardiac fibroblasts and accordingly increasing NO level.

Key words: myocardium, fibroblasts, nitric oxide synthase, nitric oxide