关键词: 药理学, 镇痛, L-硝基-精氨酸甲酯, 疼痛测定, 动物, 实验

Abstract: Objective To investigate the preliminary effects and mechanism of oxymatrine(OMT) on analgesia. Methods Mice were randomly divided into 6 groups:normal saline group, OMT groups with different doses (50,100,and 200 mg·kg^-1), pethidine hydrochloride (10 mg·kg^-1)group, and N^G-nitro-L-arginine methyl ester (L-NAME) 20 mg·kg^-1+OMT 100 mg·kg^-1 group. The number of writhing within 15 min was observed in pain mouse models caused by acetic acid. Femal e mice were randomly divided into 6 groups as the methods mentioned above. Micewere placed on the hot plate maintained at (55.0±0.5)°C and the latency of licking of the hind paws was observed. Results The number of writhing was decreased and the latency of licking of the hind paws was prolonged in a dose-pendent manner after intraperitoneal injection of OMT 50,100, and 200 mg·kg^-1 in mice(P<0.05，P<0.01，P<0.001). L-NAME injected in advance enhanced the effect of OMT on antagonism of the writhing response caused by acetic acid.Conclusion These results suggest that OMT can antagonize the acute pain caused by acetic acid and hot plate in a dose-dependent manner in mice. The analgesic action of OMT may be not mediated completely by NO-cGMP pathway.

Key words: pharmacology, analgesia, N^G<, sup>-nitro-L-arginine methyl ester (L-NAME, pain easurement, animals, laboratory