关键词: 山葡萄籽, 正交实验, 多酚, 超声波, 提取

Abstract:

Objective: To establish the optimized ultrasound-assisted extraction process of polyphenols from Vitis amurensis seeds, and to provide technical reference for the use of grape seeds.Methods: With vitis amurensis seeds as raw material and ethanol as solvent, the extraction rates of polyphenols were detected under different conditions,such as different ethanol concentrations(20%,40%,60%,80%, and 95%), extraction time(10,20,30,40,and 60 min), extraction temperatures(25℃,35℃,45℃,55℃,and 65℃), material/liguid ratios(1:4,1:8,1:10,and 1:12),extraction times(1,2,3,4,and 5) and ultrasonic powers (50,60,80,100, and 120 w) by single factor experiment and orthogonal test.Results: The optimum parameters identified were as follows:ethanol concentration was 60%,extracting temperature was 50℃,extraction time was 30 min,ultrasonic power was 120 W,material/liquid ratio was 1:8, and extracting times was 2. Under the optimal extraction conditions, the extraction rate of polyphenols from Vitis amurensis seeds was 11.71%,and the polyphenols concentration was 32.86%.Conclusion: Compared with traditional solvent extraction, ultrasound-assisted extraction of polyphenols from Vitis amurensis seeds has more advantages; the craft is simple, reasonable and efficient.

Key words: Vitis amurensis seeds, orthogonal test, polyphenols, ultrasonic wave, extraction