关键词: 前列腺癌细胞, 丁酸钠, 细胞凋亡

Abstract: To study the effect of hmSD on apoptosis of PC3 cells induced by NaBT and its mechanism.Methods PC3 cells and PC3-hmSD stablely transfected with hmSD were used as target cells.Four groups were set up:control,NaBT 2.5 mmol•L^-1,NaBT 5.0 mmol•L^-1 and NaBT 10.0 mmol•L^-1.The survival rate of cells was detected by MTT assay,apoptotic rate was determined with AO/EB assay,intracellular ROS was examined with DCFH-DA staining,the expressions of Bcl-XL,P65 and Caspase 9 were analyzed by Western blot ting method. Results ①The survival rates of PC3-hmSD cells treated with NaBT for 48 and 72h were higher than that of PC3 cells（P<0.05）. ② The apoptotic rate of PC3-hmSD cells treated with NaBT 5.0 mmol•L^-1 for　 4 h was lower than that of PC3 cells（P<0.05）.③ There was no difference of ROS between both cell lines treated with NaBT.④ The result of Western blotting showed that the expression of Bcl-XL decreased in NaBT-treated PC3 cells,but it increased in PC3-hmSD cells.Compared with control group,the expressions of P65 and Caspase 9 increased in NaBT-treated cells,but the expression of P65 increased and the expression of Caspase 9 decreased in hmSD overexpressed cells（P<0.05）. Conclusion ① NaBT can induce the apoptosis of PC3 cells,and hmSD can reverse it.② The pathway of hmSD against apoptosis may be no relation with the suppressed ROS synthesis.③ The antiapoptotic mechanism of hmSD may be involved in down-regulation of Caspase 9 and up-regulation of P65 and Bcl-XL expressions.

Key words: PC3 cell, NaBT, apoptosis