关键词: 胞二磷胆碱, 1-甲基-4-苯基吡啶, 中脑, 原代培养, 帕金森氏病

Abstract: To investigate the effect of citicoline on dopaminergic neuron injury induced by MPP⁺. Methods E14 mouse embryos were used to make mecencephalic dissociated culture.10 μmol•L^-1 MPP⁺ was used to set up the cell model of damage of dopaminergic neurons.The cell cultures were divided into control group,MPP⁺- trerated group,and another five citicoline (with different concentrations of 0.01、0.10、1.00、10.00 and 100.00 μmol•L^-1,respectively) and MPP⁺ -cotreated groups. Tyrosine hydrolysis (TH) immunochemical stainning was used to identify dopaminergic neurons.The morphological changes and the number of dopaminergic neurons were evaluated. Results On the 12th day in vitro(DIV),the number of TH-ir (TH immunal reactive) neurons was significantly reduced by MPP⁺ (P<0.05).The number of TH-ir neurons increased by 11.83% and 21.26% of control level when treated with 0.10 or 100.00 μmol•L^-1 of citicoline for 6 d respectively,compared with MPP⁺-treated alone group (P<0.05).The average length of the longest denrite of dopaminergic neurons was much longer in 0.01 μmol•L^-1and 100.00 μmol•L^-1 citicoline cotreated group,compared with MPP⁺-treated alone group(P<0.05).Conclusion Citicoline could effectively prevent the death and morphological changes of dopaminergic neurons induced by MPP⁺,and provide a new treatment way for Parkinson’s dis ease (PD.

Key words: citicoline, 1-methy1-4-pheny1-pyridinium, mesencephalon, primary cell culture, Parkison’S disease