关键词: 紫杉醇, Hep-2细胞, 细胞周期, 细胞凋亡

Abstract: Abstract:Objective To investigate the proliferation inhibition of chlorpromazine combined with taxol on Hep-2 cells (human laryngeal carcinoma cell line) and the effects on cell cycle progression. Methods Hep-2 cells at logarithmic growth phase were divided into taxol groups(3.0,6.0 and 12.0 mg•L^-1),chlorpromazine(12.0 mg•L^-1) comined with taxol (4 mg•L^-1)group and control group (100 mL culture fluid).MTT and flow cytometry were used to detect the proliferation inhibition rates of Hep-2 cells in various groups.Flow cytometry was also used to analyze the cell cycle progression of Hep-2 cells and apoptotic rate after administration.Results The proliferation inhibition rates in 3.0，6.0 and 12.0 mg•L^-1 taxol groups were 14.0%,23.9% and 36.7%，respectively,there were significant differences between three groups(P<0.05)；the proliferation inhibition rate in combined group(46.3%) was higher than those in taxol groups(P<0.05).In taxol groups.the number of G₁ phase cells decreased and the number of S and G₂ phase cells increased with the increasing of concentration of taxol.Compared with control group,the apoptotic rates in taxol groups increased(P<0.05). Conclusion Within certain concentration range,chlorpromazine could enhance taxol-induced proliferation inhibition rate,cell cycle progression and apoptotic rate of Hep-2 cells,chlorphomaxine and taxol have significant cooperation effect.

Key words: taxol, Hep-2 cell, cell cycle, apoptosis