卵巢癌组织差异蛋白的筛选及信号通路的研究

J4 ›› 2010, Vol. 36 ›› Issue (5): 912-917.

卵巢癌组织差异蛋白的筛选及信号通路的研究

徐红梅^1,2, 李春洪¹, 夏美慧¹, 孙连坤², 钟加滕², 何津¹

1.吉林大学第一医院妇产科|吉林长春 130021；2.吉林大学基础医学院病理生理学教研室|吉林长春 130021

收稿日期:2010-06-07 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 何津（Tel:0431-88782183，E-mail:hejin1204@163.com） E-mail:hejin1204@163.com
作者简介:徐红梅（1978-）|女|辽宁省大连市人|主治医师|在读医学博士|主要从事肿瘤病理生理的研究。 
基金资助:
吉林省科技厅科研基金资助课题（200705373）

Screening of differentially expressed proteins in ovarian carcinoma tissue |and research on signaling pathway

XU Hong-Mei^1,2, LI Chun-Hong¹, XIA Mei-Hui¹, SUN Lian-Kun², ZHONG Jia-Teng², HE Jin¹

1.Department |Obstetrics and Gynecology,First Hospital, Jilin University, Changchun 130021, China；2.Department of Pathophysiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China

Received:2010-06-07 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：筛查卵巢癌组织中差异蛋白，探讨这些蛋白与卵巢癌发病之间的关系。方法：收集临床正常卵巢组织标本12例作为对照组，卵巢癌组织标本19例作为实验组，提取组织蛋白，采用Pathway Array技术，用37种磷酸化抗体、73种非磷酸化抗体分析卵巢癌和正常卵巢组织中蛋白表达的差异，利用QUANTITY ONE分析软件分析图片结果计算蛋白表达量，并对结果进行统计学分析。结果：共52种蛋白有表达（47%），其中23种（21%）蛋白表达差异显著（P<0.05）。与正常卵巢组织比较，卵巢癌组织p-Stat3、p-PDK1、p-cdc2、p-p38、p-AKT、XIAP、PCNA、cdc2p34、p53、Cdk2、Mesothelin、E-cadherin蛋白表达水平明显升高（P<0.05）；而ERK、Cdc25C、Wee 1、Akt、N-cadherin、α-tubulin、Vimentin、cdc42、Bax、PTEN蛋白表达水平明显降低（P<0.05）。结合体外实验，与正常卵巢组织和正常卵巢细胞系比较，卵巢癌组织和卵巢癌细胞系中p-Stat3、p-PKD1和p-AKT表达水平明显升高（P<0.05）。结论：Stat3、PKD1和AKT作为重要信号传导分子参与了卵巢癌的发生、发展。

关键词: Pathway Array技术；差异蛋白；卵巢肿瘤

Abstract:

Abstract:Objective To detect the different proteins in ovarian carcinoma tissues and explore the relationship between the proteins and the carcinogenesis and development of ovarian carcinoma. Methods 19 cases of serous carcinoma tissues and 12 cases of normal ovarian tissues were collected, the proteins from tissues were extracted. With protein pathway array technique,37 kinds of phosphorylative antibodies and 73 kinds of non-phosphorylative antibodies, the different proteins in ovarian carcinoma tissues and normal ovarian tissues were detected. The results were analyzed by QUANTITY One software and statistically calculated. Results There were 52 proteins expressed (47%) and 23 proteins (21%) were diferentially expressed with statistical significance (P<0.05), including the over expression proteins: p-Stat3, p-PDK1, p-cdc2, p-p38, p-AKT, XIAP, PCNA, cdc2p34, p53, Cdk2, Mesothelin, E-cadherin and down-regulated proteins: ERK, Cdc25C, Wee 1, Akt, N-cadherin, α-tubulin, Vimentin, cdc42, Bax, PTEN (P<0.05). Compared with normal ovarian tissues and normal ovarian cell line,p-Stat3, p-PKD1 and p-AKT were over expressed in ovarian carcinoma tissues and cell lines (P<0.05). Conclusion Stat3, PKD1 and AKT play important roles in carcinogenesis of ovarian carcinoma

Key words: Pathway Array technique, differentially expressed proteins, ovarian neoplasms

中图分类号:

R737.31

徐红梅, 李春洪, 夏美慧, 孙连坤, 钟加滕, 何津. 卵巢癌组织差异蛋白的筛选及信号通路的研究[J]. J4, 2010, 36(5): 912-917.

XU Hong-Mei, LI Chun-Hong, XIA Mei-Hui, SUN Lian-Kun, ZHONG Jia-Teng, HE Jin. Screening of differentially expressed proteins in ovarian carcinoma tissue |and research on signaling pathway[J]. J4, 2010, 36(5): 912-917.

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