高氧肺损伤肺组织中IL-8和IL-10含量的变化及意义

J4 ›› 2010, Vol. 36 ›› Issue (5): 918-921.

高氧肺损伤肺组织中IL-8和IL-10含量的变化及意义

李玉梅¹, 戴春来², 周文莉¹, 严超英¹

1. 吉林大学第一医院儿科|吉林 |长春130021; |2. 吉林大学第一医院影像科|吉林 |长春130021

收稿日期:2010-06-16 出版日期:2010-09-28 发布日期:2010-09-28
通讯作者: 严超英(Tel: 0431-88782771, E-mail:yanchaoying224@126.com ) E-mail:yanchaoying224@126.com
作者简介:李玉梅（1969-）|女|吉林省磐石市人|副主任医师|副教授|医学博士|主要从事小儿危重症呼吸损害的研究。
基金资助:
吉林省科技厅科研基金资助课题（201015158,200705109 ）

Changes of IL-8 and IL-10 in lung tissues with hyperoxia-induced injury

LI Yu-Mei¹, DAI Chun-Lai², ZHOU Wen-Li¹, YAN Chao-Ying¹

1.Department of Pediatrics, First Hospital, Jilin University, Changchun 130021,China;2.Department of Radiology, First Hospital, Jilin University, Changchun 130021,China

Received:2010-06-16 Online:2010-09-28 Published:2010-09-28

摘要/Abstract

摘要：

目的：研究高氧肺损伤大鼠肺组织中细胞因子IL-8和IL-10的变化，探讨其在高氧肺损伤中的作用。方法：吸入＞95%氧气建立高氧肺损伤大鼠模型，于给氧后6 h，1、3、6、10、14 d留取肺组织标本，HE染色观察肺组织病理形态学改变，并应用双抗体夹心ELISA法检测肺组织匀浆IL-8和IL-10的含量。结果：随给氧时间延长高氧组肺泡腔内出现炎性细胞浸润、肺泡腔增大、肺泡间隔较薄、肺泡数量减少。与空气组比较，高氧组肺组织匀浆中IL-8含量很快升高，在3 d时达到高峰，这种差异持续至生后14 d，高氧组IL-10含量的升高在给氧后6 h时明显，与对照组比较差异有显著性(P<0.05)，1 d时仍有差异，约3d即恢复正常。结论：高氧肺损伤时炎症因子IL-8参与炎症反应迅速而持久，而抗炎因子IL-10持续时间短暂。

关键词: 肺损伤；高氧症；白细胞介素8；白细胞介素10

Abstract:

Abstract:Objective To study the changes of interleukin 8 (IL-8) and interleukin 10 (IL-10) in hyperoxia-induced lung injury and significances. Methods The model of rat with hyperoxia-induced lung injury was established by being inhaled with high concentration of oxygen (>95%).The lung tissues were harvested 6 h and 1,3,6,10,14 d after oxygen treatment, the histopathological changes of lung tissues were observed by HE staining and Double-antigen sandwich ELISA was used to detect the IL-8 and IL-10 levels in the lung tissue homogenate. Results The infiltrative inflammatory cells appeared in alveolus first,and then alveolus was enlarged ,then alveolar septum became thinner and eventually the number of alveolus was decreased as the time of exposure to oxygen prolonged. The concentrations of IL-8 in lung tissue homogenate in high oxygen group were significantly higher than those in control group, the level reached the peak at day 3 after high oxygen exposure and the statistical difference lasted until the 14th day. The concentrations of IL-10 in lung tissue homogenate were significantly higher after high oxygen exposure 6 h and also had statistical difference on day 1, but restored normal level at day 3. Conclusion The concentration of inflammatory factor IL-8 is increased fast and lasts for long time while anti-inflammatory factor IL-10 is increased for short time in hyperoxia-induced lung injury.

Key words: lung injury;hyperoxia;interleukin　8;interleukin 10

中图分类号:

R722.19

李玉梅, 戴春来, 周文莉, 严超英. 高氧肺损伤肺组织中IL-8和IL-10含量的变化及意义[J]. J4, 2010, 36(5): 918-921.

LI Yu-Mei, DAI Chun-Lai, ZHOU Wen-Li, YAN Chao-Ying. Changes of IL-8 and IL-10 in lung tissues with hyperoxia-induced injury[J]. J4, 2010, 36(5): 918-921.