胰腺源间充质干细胞对大鼠胰腺损伤的修复作用

doi:国家自然科学基金资助课题（39970741）；吉

胰腺源间充质干细胞对大鼠胰腺损伤的修复作用

叶海青¹，范东燕²，刘雅娟²，陈强²，范洪学²

1.吉林大学农学部食品质量与安全系，吉林长春 130062；2.吉林大学公共卫生学院卫生毒理学教研室，吉林长春 130021

收稿日期:2007-10-20 修回日期:1900-01-01 出版日期:2008-07-28 发布日期:2008-07-28
通讯作者: 范洪学

Repairment of pancreas mesenchymal stem cells on injured pancreas tissues

YE Hai-qing¹,FAN Dong-yan²,LIU Ya-juan²,CHEN Qiang²,FAN Hong-xue²

1.Department of Food Quality and Safety,School of Agriculture,Jilin University,Changchun 130062,China;2.Department of Health Toxicology,School of Public Health,Jilin University,Changchun 130021,China

Received:2007-10-20 Revised:1900-01-01 Online:2008-07-28 Published:2008-07-28
Contact: FAN Hong-xue

摘要/Abstract

摘要： 目的：探讨从大鼠胰腺中分离出间充质干细胞（MSCs），并用于大鼠胰腺损伤的修复，为糖尿病治疗探寻新的细胞来源。方法：①取3日龄Wistar大鼠，无菌条件取出胰腺，通过Ⅴ型胶原酶消化获得细胞，常规培养传代、贴壁筛选法纯化细胞、Giemsa染色观察细胞形态、流式细胞仪测定表面标志CD34和CD44，同时与骨髓MSCs进行对照，观察两者在性质和作用上的异同。②实验大鼠随机分为实验组（20只）和对照组（20只）。结扎大鼠胰腺组织建立胰腺组织缺血性坏死模型，将分离、纯化的胰腺MSCs用DAPI（荧光）标记移植到实验组坏死胰腺，对照组移植稀释液。2周后统计两组生存率并做组织病理学检测。结果：①随培养时间延长，细胞形态驱于一致，增殖旺盛。形态学观察和表面抗原检测与骨髓MSCs具有相似性。②实验组大鼠存活率为75%，肉眼见坏死的胰腺组织外观基本恢复，组织病理学切片可见组织细胞结构完整。在荧光显微镜下可见到标记DAPI的蓝色细胞。对照组的大鼠存活率20％，解剖见腹腔大量渗水，组织粘连。实验组存活率大于对照组（P<0.05）。结论：胰腺中存在MSCs，可在体外培养、扩增并可用于修复损伤的胰腺组织。

关键词: 间充质干细胞, 体外扩增, 移植, 修复

Abstract: To study the repairment of pancreas mesenchymal stem cells(MSCs) on injured pancreas of rats,and find a new source of cells for treatment of diabetes. Methods ① Pancreases were taken out of three-day-old Wistar rats under bioclean condition,and cells were obtained by V-collagenase digestion. Generations were passed by conventional culture,cells were purified by adherence screening method,cell morphous was observed by Giemsa staining. The expressions of surface marker CD34 and CD44 were determined by FCM,and compared with bone marrow MSCs(BM-MSCs),the differences in character and function were observed. ②The experimental rats were divided into two groups randomly. Pancreas ischemic necrosis model was made by deligation,then the purified pancreas MSCs were marked with DAPI and then were transplantated partially. After two weeks,the survival rate was measured and histopathological detection was performed. Results ① The cells had concordant morphous gradually with vigorous generation. There was no significant difference in morphology and surface antigen compared with BM-MSCs.② The survival rate in experimental group was 75% ，the necrotic tissue had basi-rebounded. Blue fluorescent was observed in repaired pancreas tissues. The survival rate in control group was 20%. The survival rate in experimental group was higher than that in control group (P<0.05). Conclusion There are MSCs in pancreas which can be cultivated in vitro and has the function of amplification as well as be used to repair injured pancreas.

Key words: mesenchymal stem cells, amplification in vitro, transplantation, repair

中图分类号:

R-332

叶海青，范东燕，刘雅娟，陈强，范洪学. 胰腺源间充质干细胞对大鼠胰腺损伤的修复作用[J]. J4, 2008, 34(4): 551-555.

YE Hai-qing,FAN Dong-yan,LIU Ya-juan,CHEN Qiang,FAN Hong-xue. Repairment of pancreas mesenchymal stem cells on injured pancreas tissues[J]. J4, 2008, 34(4): 551-555.

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