关键词: 神经保护作用, 谷氨酸, 神经元

Abstract: To investigate the effects of antipsychotic olanzapine on the survival rate of neurons,LDH release,percentage of apoptotic cells and death rate of the hippocampus neurons injured by glutamate. Methods Primary　 cultured hippocampal neurons were injured with glutamate. Three groups were divided as control,glutamate insult and olanzapine administration groups. The survival rate of neurons was detected with MTT method and LDH release was detected by spectrophometry. The changes in percentage of apoptotic cells and death rate of neurons were detected respectively by FCM before and after administration with olanzapine. Results The　 survival rate of neurons injured with the excitatory neurotoxicity of glutamate decreased to 50% of control (P<0.05). The survival rates of neurons injured with glutamate increased dramatically after administration with olanzapine as compared with those in glutamate group (P<0.05 or P<0.01). The LDH release of neurons injured with glutamate increased significantly,which was 7.4-fold of control group,however,LDH release from neurons injured with glutamate decreased after administration with olanzapine as compared with that in glutamate group. The percentages of apoptotic cells of neurons injured with glutamate increased (P<0.01 ),but oalnzapine can reverse it. The death rates of neurons injured with glutamate increased compared with control (P<0.05 ),nevertheless,the administration of olanzapine reduced the death rates of neurons injured with glutamate as compared with that in glutamate group (P<0.05,P<0.01). Conclusion Olanzapine can prevent the hippocampal neurons from the damage induced with glutamate,elevate the neuron survival rate,reduce the LDH release,keep the cell membrane intact,and decrease the percentage of apoptotic cells and death rate of neurons,which has the neuroprotection.

Key words: neuroprotection, glutamic acid, neurons