关键词: 细胞周期, 解偶联, Jurkat细胞, EL-4细胞

Abstract: To observe the effect of 5-flurouracil (5-FU) with different doses and administration time on cell cycle uncoupling of Jurkat and EL-4 cell lines.Methods The dose- and time-effects of cell cycle were detected by flow cytometry (FCM) following staining of Jurkat and EL-4 cells with propidium iodide (PI) and the changes of polyploid cells were observed after treatment with 5-FU with different doses (0,0.001,0.010,0.100 and 1.000 mg•L^-1) 　and administration time (0,4,8,16,24 and 48 h). Results As compared with 0 mg•L^-1group,the percentages of 8N cells were decreased significantly 16 h after exposure of Jurkat cell line to 5-FU with doses of 0.001,0.010,0.100 and 1.000 mg•L^-1 (P<0.001). After exposure of EL-4 cell line to 5-FU with doses of 0.010,0.100 mg•L^-1,the percentages of 8N cells were increased significantly compared with 0 mg•L^-1 group (P<0.05). As compared with control,the percentages of 8N cells were decreased at 8,16 and 24 h and increased significantly at 48 h after exposure of Jurkat cell line to 5-FU with dose of 0.100 mg•L^-1 (P<0.01). And the percentages of 8N cells were increased at 4,8 and 16 h and decreased significantly at 48 h after exposure of EL-4 cell line to 5-FU with dose of 0.100 mg•L^-1 (P<0.05,P<0.01). Conclusion The cell cycle uncoupling of EL-4 cell line could be induced by 5-FU,but Jurkat cell line could not be influenced.

Key words: cell cycle, uncoupling, Jurkat cell line, EL-4 cell line