关键词: 人工骺板软骨, 骺板损伤, 动物, 实验

Abstract: Objective To investigate the possibility of repairing the epiphyseal plate injury using artificial epiphyseal plate.Methods The left tibial epiphyseal plate of rabbit was resected about 1/3 to 1/2, then the artificial epiphyseal plate were transplanted; while the right side was injured but not proceed transplantation as contrast. The tibial angle was measured after X-ray check. T test was used for data analyzing. HE stain, toluidine blue Dyeing and immunohistochemistry test were performed to observe the histomorphology. Results: The tibial angle of lower extremity was (38.80±13.50)° in control group, (9.01±4.2)° in artificial epiphyseal plate group 8 weeks after transplantation; (54.25±18.33)° in control group, (11.27±5.1)° in artificial epiphyseal plate group 24 weeks after transplantation. There was significantly difference between control group and artificial epiphyseal plate group (P<0.01). There was bony bridge formation in control group. 2 weeks after planting, the epiphyseal plate impairment region was filled with cartilage tissue, but the chondrocytes in the transplanted epiphyseal plate were changed into irregular arrangement, and demarcation was distinct with normal epiphyseal plate cartilage. After planting for 4 weeks, cartilage layers were noted in the treatment groups and the cutting edges disappeared. There were collagen-Ⅱ-positive cells in transplanted epiphyseal plate cartilage.Conclusion The artificial epiphyseal plate can repair the epiphyseal plate injury, and the effect is satisfied.

Key words: artificial epiphyseal plate cartilage, epiphyseal plate injury, animal, laboratory