关键词: 细胞凋亡, 耐药MG-63细胞

Abstract: To find the molecular mechanism of apoptosis of multi-drug resistance cells lines(MDR-MG-63) induced by semiconductor laser and provide theoretical basis for its clinical application. Methods MDR-MG-63 cells were divided into five groups:control group and semiconductor laser groups(30,60,90,120J•cm^-2).The cell viability was detected by MTT assay; reactive oxygen species (ROS) was detected by flow cytometry;the protein expressions of caspase-8,caspase-9 and caspase-3 were assayed by Western blotting. Results Compared with control group,the cell viabilities decreased gradually with the increasing of dose in semiconductor laser groups (60,90,120J•cm^-2)(P<0.05 or P<0.01),a lot of ROS were found (P<0.05) after multi-drug resistance MG-63 cells lines were irradiated,at the same time the expression levels of caspase-9 protein and caspase-3 protein were increased(P<0.05 or P<0.01) in a dose-dependent manner,but the level of caspase-8 protein didn’[KG-*5]t obviously change （P>0.05）;the differences of the cell viability,ROS,the expression levels of caspase-9 protein and caspase-3 protein between semiconductor laser(30J•cm^-2)group and control group were not significant (P>0.05).Conclusion Semiconductor laser can induce apoptosis of MG-63 cells by mitochondrion pathway,and the process may be associated with the increasing of ROS.

Key words: apoptosis, multi-drug resistance MG-63 cells